|
| 1 | +.. include:: ../references.txt |
| 2 | + |
| 3 | +.. _image_analysis_tutorial: |
| 4 | + |
| 5 | +*********************** |
| 6 | +Image Analysis Tutorial |
| 7 | +*********************** |
| 8 | + |
| 9 | +Due to the high electron cross-section, background signals (or baseline) are |
| 10 | +much more of a problem for electron diffraction than equivalent X-ray experiments. |
| 11 | + |
| 12 | +Contents |
| 13 | +======== |
| 14 | + |
| 15 | +* :ref:`streaming` |
| 16 | +* :ref:`powder` |
| 17 | + |
| 18 | +.. _streaming: |
| 19 | + |
| 20 | +Streaming Image Processing |
| 21 | +========================== |
| 22 | + |
| 23 | +Diffraction datasets can be large, much larger than a machine can handle |
| 24 | +in-memory. In this case, it makes sense to assemble processing pipelines instead |
| 25 | +of working on the data all at once. |
| 26 | + |
| 27 | +Consider the following snippet to combine 50 images |
| 28 | +from an iterable :code:`source`:: |
| 29 | + |
| 30 | + import numpy as np |
| 31 | + |
| 32 | + images = np.empty( shape = (2048, 2048, 50) ) |
| 33 | + from index, im in enumerate(source): |
| 34 | + images[:,:,index] = im |
| 35 | + |
| 36 | + avg = np.average(images, axis = 2) |
| 37 | + |
| 38 | +If the :code:`source` iterable provided 1000 images, the above routine would |
| 39 | +not work on most machines. Moreover, what if we want to transform the images |
| 40 | +one by one before averaging them? What about looking at the average while it |
| 41 | +is being computed? |
| 42 | + |
| 43 | +Scikit-ued provides some functions that can make streaming processing possible. These |
| 44 | +function will have an 'i' prefix (for :code:`iterator`). Let's look at an example:: |
| 45 | + |
| 46 | + import numpy as np |
| 47 | + from skued.image import ialign, iaverage |
| 48 | + from skimage.io import imread |
| 49 | + |
| 50 | + stream = map(imread, list_of_filenames) |
| 51 | + aligned = ialign(stream) |
| 52 | + averaged = iaverage(aligned) |
| 53 | + |
| 54 | +At this point, the generators :code:`map`, :code:`ialign`, and :code:`iaverage` are 'wired' |
| 55 | +but will not compute anything until it is requested. We can use the function |
| 56 | +:code:`last` to get at the final average, but we could also look at the average |
| 57 | +step-by-step by calling :code:`next`:: |
| 58 | + |
| 59 | + avg = next(averaged) # only one images is loaded, aligned and added to the average |
| 60 | + total = last(averaged) # average of the entire stream |
| 61 | + |
| 62 | +An important advantage of processing images in a streaming fashion is the lower |
| 63 | +memory usage; this allows the use of multiple processes in parallel:: |
| 64 | + |
| 65 | + from skued import pmap |
| 66 | + |
| 67 | + def align_and_avg(filenames): |
| 68 | + stream = map(imread, filenames) |
| 69 | + aligned = ialign(stream) |
| 70 | + return last(iaverage(aligned)) |
| 71 | + |
| 72 | + batches = [list_of_filenames1, list_of_filenames2, list_of_filenames3] |
| 73 | + |
| 74 | + for avg in pmap(align_and_avg, batches, processes = 3): |
| 75 | + # write to disk of display |
| 76 | + pass |
| 77 | + |
| 78 | +Example: averaging with error |
| 79 | +------------------------------ |
| 80 | + |
| 81 | +It is possible to combine :code:`iaverage` and :code:`isem` into a single stream using :code:`itertools.tee`. |
| 82 | +Here is a recipe for it:: |
| 83 | + |
| 84 | + def iaverage_with_error(images, weights): |
| 85 | + """ |
| 86 | + Combined streaming average and standard error of diffraction images. |
| 87 | + |
| 88 | + Parameters |
| 89 | + ---------- |
| 90 | + images : iterable of ndarrays |
| 91 | + Images to be averaged. This iterable can also a generator. |
| 92 | + weights : iterable of ndarray, iterable of floats, or None, optional |
| 93 | + Array of weights. See `numpy.average` for further information. If None (default), |
| 94 | + total picture intensity of valid pixels is used to weight each picture. |
| 95 | + |
| 96 | + Yields |
| 97 | + ------ |
| 98 | + avg: `~numpy.ndarray` |
| 99 | + Weighted average. |
| 100 | + sem: `~numpy.ndarray` |
| 101 | + Weighted average. |
| 102 | + """ |
| 103 | + stream1, stream2 = itertools.tee(images, n = 2) |
| 104 | + averages = iaverage(stream1, weights = weights) |
| 105 | + errors = isem(stream2) |
| 106 | + yield from zip(averages, errors) |
| 107 | + |
| 108 | +.. _powder: |
| 109 | + |
| 110 | +Image analysis on polycrystalline diffraction patterns |
| 111 | +====================================================== |
| 112 | + |
| 113 | +Center-finding |
| 114 | +-------------- |
| 115 | +Polycrystalline diffraction patterns display concentric rings, and finding |
| 116 | +the center of those concentric rings is important. |
| 117 | + |
| 118 | +Let's load a test image:: |
| 119 | + |
| 120 | + from skimage import img_as_uint |
| 121 | + from skimage.io import imread |
| 122 | + import matplotlib.pyplot as plt |
| 123 | + |
| 124 | + path = '\\data\\vo2.tif' |
| 125 | + im = img_as_uint(imread(path, plugin = 'tifffile')) |
| 126 | + |
| 127 | + mask = np.zeros_like(im, dtype = np.bool) |
| 128 | + mask[0:1250, 700:1100] = True |
| 129 | + im[mask] = 0 |
| 130 | + |
| 131 | + plt.imshow(im, vmin = 1000, vmax = 1200) |
| 132 | + plt.show() |
| 133 | + |
| 134 | +.. plot:: |
| 135 | + |
| 136 | + from skimage import img_as_uint |
| 137 | + from skimage.io import imread |
| 138 | + import matplotlib.pyplot as plt |
| 139 | + path = 'data\\vo2.tif' |
| 140 | + im = img_as_uint(imread(path, plugin = 'tifffile')) |
| 141 | + mask = np.zeros_like(im, dtype = np.bool) |
| 142 | + mask[0:1250, 700:1100] = True |
| 143 | + im[mask] = 0 |
| 144 | + plt.imshow(im, vmin = 1000, vmax = 1200) |
| 145 | + plt.show() |
| 146 | + |
| 147 | +This is a noisy diffraction pattern of polycrystalline vanadium dioxide. |
| 148 | +Finding the center of such a symmetry pattern can be done with the |
| 149 | +:code:`powder_center` routine:: |
| 150 | + |
| 151 | + from skued.image import powder_center |
| 152 | + ic, jc = powder_center(im, mask = mask) |
| 153 | + |
| 154 | + # Plotting the center as a black disk |
| 155 | + import numpy as np |
| 156 | + ii, jj = np.meshgrid(np.arange(im.shape[0]), np.arange(im.shape[1]), |
| 157 | + indexing = 'ij') |
| 158 | + rr = np.sqrt((ii - ic)**2 + (jj - jc)**2) |
| 159 | + im[rr < 100] = 0 |
| 160 | + |
| 161 | + plt.imshow(im, vmax = 1200) |
| 162 | + plt.show() |
| 163 | + |
| 164 | +.. plot:: |
| 165 | + |
| 166 | + from skimage import img_as_uint |
| 167 | + from skimage.io import imread |
| 168 | + import numpy as np |
| 169 | + import matplotlib.pyplot as plt |
| 170 | + path = 'data\\vo2.tif' |
| 171 | + im = img_as_uint(imread(path, plugin = 'tifffile')) |
| 172 | + from skued.image import powder_center |
| 173 | + mask = np.zeros_like(im, dtype = np.bool) |
| 174 | + mask[0:1250, 700:1100] = True |
| 175 | + ic, jc = powder_center(im, mask = mask) |
| 176 | + ii, jj = np.meshgrid(np.arange(im.shape[0]), np.arange(im.shape[1]),indexing = 'ij') |
| 177 | + rr = np.sqrt((ii - ic)**2 + (jj - jc)**2) |
| 178 | + im[rr < 100] = 0 |
| 179 | + plt.imshow(im, vmin = 1000, vmax = 1200) |
| 180 | + plt.show() |
| 181 | + |
| 182 | +Angular average |
| 183 | +--------------- |
| 184 | + |
| 185 | +First, we create a test image:: |
| 186 | + |
| 187 | + import numpy as np |
| 188 | + import matplotlib.pyplot as plt |
| 189 | + from skued import gaussian |
| 190 | + |
| 191 | + image = np.zeros( (256, 256) ) |
| 192 | + xc, yc = image.shape[0]/2, image.shape[1]/2 # center |
| 193 | + |
| 194 | + extent = np.arange(0, image.shape[0]) |
| 195 | + xx, yy = np.meshgrid(extent, extent) |
| 196 | + rr = np.sqrt((xx - xc)**2 + (yy-yc)**2) |
| 197 | + image += gaussian([xx, yy], center = [xc, yc], fwhm = 200) |
| 198 | + image[np.logical_and(rr < 40, rr > 38)] = 1 |
| 199 | + image[np.logical_and(rr < 100, rr > 98)] = 0.5 |
| 200 | + image /= image.max() # Normalize max to 1 |
| 201 | + image += np.random.random(size = image.shape) |
| 202 | + |
| 203 | + plt.imshow(image) |
| 204 | + plt.show() |
| 205 | + |
| 206 | +.. plot:: |
| 207 | + |
| 208 | + import numpy as np |
| 209 | + import matplotlib.pyplot as plt |
| 210 | + from skued import gaussian |
| 211 | + image = np.zeros( (256, 256) ) |
| 212 | + xc, yc = image.shape[0]/2, image.shape[1]/2 # center |
| 213 | + extent = np.arange(0, image.shape[0]) |
| 214 | + xx, yy = np.meshgrid(extent, extent) |
| 215 | + rr = np.sqrt((xx - xc)**2 + (yy-yc)**2) |
| 216 | + image += gaussian([xx, yy], center = [xc, yc], fwhm = 200) |
| 217 | + image[np.logical_and(rr < 40, rr > 38)] = 1 |
| 218 | + image[np.logical_and(rr < 100, rr > 98)] = 0.5 |
| 219 | + image /= image.max() # Normalize max to 1 |
| 220 | + image += np.random.random(size = image.shape) |
| 221 | + plt.imshow(image) |
| 222 | + plt.show() |
| 223 | + |
| 224 | + |
| 225 | +... and we can easily compute an angular average:: |
| 226 | + |
| 227 | + from skued.image import angular_average |
| 228 | + |
| 229 | + radius, intensity = angular_average(image, (xc, yc)) |
| 230 | + |
| 231 | + plt.plot(radius, intensity) |
| 232 | + |
| 233 | +.. plot:: |
| 234 | + |
| 235 | + from skued.image import angular_average |
| 236 | + from skued import gaussian |
| 237 | + import numpy as np |
| 238 | + import matplotlib.pyplot as plt |
| 239 | + from skued import gaussian |
| 240 | + image = np.zeros( (256, 256) ) |
| 241 | + xc, yc = image.shape[0]/2, image.shape[1]/2 # center |
| 242 | + extent = np.arange(0, image.shape[0]) |
| 243 | + xx, yy = np.meshgrid(extent, extent) |
| 244 | + rr = np.sqrt((xx - xc)**2 + (yy-yc)**2) |
| 245 | + image += gaussian([xx, yy], center = [xc, yc], fwhm = 200) |
| 246 | + image[np.logical_and(rr < 40, rr > 38)] = 1 |
| 247 | + image[np.logical_and(rr < 100, rr > 98)] = 0.5 |
| 248 | + image /= image.max() # Normalize max to 1 |
| 249 | + image += np.random.random(size = image.shape) |
| 250 | + radius, intensity = angular_average(image, (xc, yc)) |
| 251 | + plt.plot(radius, intensity) |
| 252 | + plt.show() |
| 253 | + |
| 254 | +:ref:`Return to Top <image_analysis_tutorial>` |
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