diff --git a/VERSIONLOG.md b/VERSIONLOG.md
index 1041690f..c1fe31b2 100644
--- a/VERSIONLOG.md
+++ b/VERSIONLOG.md
@@ -1,5 +1,9 @@
 # Scilifelab_epps Version Log
 
+## 20251113.1
+
+Set default to false for file removal in file upload, pass parameter as false in aviti samplesheet generation just to be on the safe side
+
 ## 20251105.1
 
 Fix bugs in ONT couchdb connections
diff --git a/scilifelab_epps/epp.py b/scilifelab_epps/epp.py
index 498f8759..3927a75e 100644
--- a/scilifelab_epps/epp.py
+++ b/scilifelab_epps/epp.py
@@ -558,7 +558,7 @@ def upload_file(
     file_slot: str,
     process: Process,
     lims: Lims,
-    remove: bool = True,
+    remove: bool = False,
     fail_on_missing_file_slot: bool = True,
 ):
     matching_file_slots = [
diff --git a/scripts/generate_aviti_run_manifest.py b/scripts/generate_aviti_run_manifest.py
index 98edc5da..889b0e6d 100644
--- a/scripts/generate_aviti_run_manifest.py
+++ b/scripts/generate_aviti_run_manifest.py
@@ -583,6 +583,7 @@ def main(args: Namespace):
         args.file,
         process,
         lims,
+        remove=False,
     )
 
     # Move manifest(s)