diff --git a/examples/PXD042173/PXD042173.sdrf.tsv b/examples/PXD042173/PXD042173.sdrf.tsv new file mode 100644 index 00000000..c3d229eb --- /dev/null +++ b/examples/PXD042173/PXD042173.sdrf.tsv @@ -0,0 +1,178 @@ +source name characteristics[organism] characteristics[organism part] characteristics[cell type] characteristics[disease] characteristics[age] characteristics[sex] characteristics[biological replicate] characteristics[material type] characteristics[sample type] characteristics[enrichment process] characteristics[crosslink distance] characteristics[crosslinking reaction time] characteristics[crosslinking temperature] assay name technology type comment[data file] comment[technical replicate] comment[fraction identifier] comment[label] comment[instrument] comment[proteomics data acquisition method] comment[cleavage agent details] comment[cleavage agent details] comment[modification parameters] comment[modification parameters] comment[dissociation method] comment[collision energy] comment[precursor mass tolerance] comment[fragment mass tolerance] comment[fractionation method] comment[chemical cross-linking coupled with ms] comment[cross-linker] comment[crosslink enrichment method] comment[crosslinker concentration] comment[quenching reagent] comment[reduction reagent] comment[alkylation reagent] comment[sdrf version] comment[sdrf template] comment[sdrf template] comment[sdrf template] +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210727_MxR_FDR_firstbatch_DSSOplate2_B12 proteomic profiling by mass spectrometry L1_20210727_MxR_FDR_firstbatch_DSSOplate2_B12.raw 1 1 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210727_MxR_FDR_firstbatch_DSSOplate2_E12 proteomic profiling by mass spectrometry L1_20210727_MxR_FDR_firstbatch_DSSOplate2_E12.raw 1 2 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210727_MxR_FDR_firstbatch_DSSOplate2_H12 proteomic profiling by mass spectrometry L1_20210727_MxR_FDR_firstbatch_DSSOplate2_H12.raw 1 3 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_C10 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_C10.raw 1 4 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_C11 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_C11.raw 1 5 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_C12 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_C12.raw 1 6 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_C6 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_C6.raw 1 7 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_C7 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_C7.raw 1 8 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_C8 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_C8.raw 1 9 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_C9 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_C9.raw 1 10 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_D1 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_D1.raw 1 11 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_D2 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_D2.raw 1 12 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_D3 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_D3.raw 1 13 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_D4 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_D4.raw 1 14 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_D5 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_D5.raw 1 15 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_D6 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_D6.raw 1 16 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_D7 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_D7.raw 1 17 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210806_MxR_FDR_firstbatch_DSSOplate_D8 proteomic profiling by mass spectrometry L1_20210806_MxR_FDR_firstbatch_DSSOplate_D8.raw 1 18 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_D10_remeasure proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_D10_remeasure.raw 1 19 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_D11 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_D11.raw 1 20 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_D12 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_D12.raw 1 21 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_D9_remeasure proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_D9_remeasure.raw 1 22 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_E10 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_E10.raw 1 23 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_E11 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_E11.raw 1 24 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_E12 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_E12.raw 1 25 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_E1_remeasure proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_E1_remeasure.raw 1 26 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_E2 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_E2.raw 1 27 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_E3 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_E3.raw 1 28 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_E4 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_E4.raw 1 29 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_E5 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_E5.raw 1 30 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_E6 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_E6.raw 1 31 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_E7 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_E7.raw 1 32 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_E8 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_E8.raw 1 33 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_E9 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_E9.raw 1 34 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_F1 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_F1.raw 1 35 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_F2 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_F2.raw 1 36 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch1 homo sapiens not applicable not applicable not applicable not available not applicable 1 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20210811_MxR_FDR_firstbatch_DSSOplate_F3 proteomic profiling by mass spectrometry L1_20210811_MxR_FDR_firstbatch_DSSOplate_F3.raw 1 37 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_B10 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_B10.raw 1 1 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_B11 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_B11.raw 1 2 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_B12 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_B12.raw 1 3 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_B1_B2 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_B1_B2.raw 1 4 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_B3_B4 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_B3_B4.raw 1 5 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_B5_B6 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_B5_B6.raw 1 6 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_B7_B8 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_B7_B8.raw 1 7 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_B9 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_B9.raw 1 8 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_C1 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_C1.raw 1 9 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_C2 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_C2.raw 1 10 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_C3 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_C3.raw 1 11 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_C4_152127 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_C4_152127.raw 1 12 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_C4_212733 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_C4_212733.raw 1 13 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_C5_152127 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_C5_152127.raw 1 14 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_C5_212733 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_C5_212733.raw 1 15 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_C6 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_C6.raw 1 16 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_C7 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_C7.raw 1 17 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_C8 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_C8.raw 1 18 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_C9_C10 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_C9_C10.raw 1 19 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_D11_D12 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_D11_D12.raw 1 20 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_D1_D2 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_D1_D2.raw 1 21 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_D3_D4 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_D3_D4.raw 1 22 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_D5_D6 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_D5_D6.raw 1 23 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_D7_D8 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_D7_D8.raw 1 24 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_D9_D10 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_D9_D10.raw 1 25 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_E11_E12 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_E11_E12.raw 1 26 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_E1_E2 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_E1_E2.raw 1 27 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_E3_E4 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_E3_E4.raw 1 28 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_E5_E6 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_E5_E6.raw 1 29 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_E7_E8 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_E7_E8.raw 1 30 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_E9_E10 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_E9_E10.raw 1 31 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_F11_F12 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_F11_F12.raw 1 32 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_F1_F2 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_F1_F2.raw 1 33 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_F3_F4 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_F3_F4.raw 1 34 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_F5_F6 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_F5_F6.raw 1 35 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_F7_F8 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_F7_F8.raw 1 36 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch2 homo sapiens not applicable not applicable not applicable not available not applicable 2 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211004_MxR_secondbatch_DSSOplate_F9_F10 proteomic profiling by mass spectrometry L1_20211004_MxR_secondbatch_DSSOplate_F9_F10.raw 1 37 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_B11_B12 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_B11_B12.raw 1 1 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_B7_B8 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_B7_B8.raw 1 2 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_B9_B10 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_B9_B10.raw 1 3 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_C11_C12_212733 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_C11_C12_212733.raw 1 4 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_C1_C2 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_C1_C2.raw 1 5 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_C3_C4 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_C3_C4.raw 1 6 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_C5_C6 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_C5_C6.raw 1 7 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_C7_C8 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_C7_C8.raw 1 8 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_C9_C10 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_C9_C10.raw 1 9 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_D11_D12 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_D11_D12.raw 1 10 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_D1_D2 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_D1_D2.raw 1 11 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_D3_D4 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_D3_D4.raw 1 12 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_D5_D6 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_D5_D6.raw 1 13 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_D7_D8 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_D7_D8.raw 1 14 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_D9_D10 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_D9_D10.raw 1 15 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_E11_E12 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_E11_E12.raw 1 16 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_E1_E2 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_E1_E2.raw 1 17 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_E3_E4_212733 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_E3_E4_212733.raw 1 18 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_E5_E6 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_E5_E6.raw 1 19 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_E7_E8 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_E7_E8.raw 1 20 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_E9_E10 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_E9_E10.raw 1 21 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_F1_F2 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_F1_F2.raw 1 22 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_F3_F4 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_F3_F4.raw 1 23 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch3 homo sapiens not applicable not applicable not applicable not available not applicable 3 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20211118_MxR_thirdbatch_DSSOplate_F5_F6 proteomic profiling by mass spectrometry L1_20211118_MxR_thirdbatch_DSSOplate_F5_F6.raw 1 24 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_B11_B12 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_B11_B12.raw 1 1 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_B1_B2 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_B1_B2.raw 1 2 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_B3_B4 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_B3_B4.raw 1 3 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_B5_B6 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_B5_B6.raw 1 4 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_B7_B8 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_B7_B8.raw 1 5 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_B9_B10 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_B9_B10.raw 1 6 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_C11_C12 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_C11_C12.raw 1 7 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_C1_C2 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_C1_C2.raw 1 8 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_C3_C4 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_C3_C4.raw 1 9 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_C5_C6 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_C5_C6.raw 1 10 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_C7_C8 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_C7_C8.raw 1 11 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_C9_C10 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_C9_C10.raw 1 12 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_D11_D12 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_D11_D12.raw 1 13 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_D1_D2 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_D1_D2.raw 1 14 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_D3_D4 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_D3_D4.raw 1 15 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_D5_D6 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_D5_D6.raw 1 16 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_D7_D8 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_D7_D8.raw 1 17 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_D9_D10 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_D9_D10.raw 1 18 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220110_MxR_FDR_fourthbatch_DSSOplate_E7_E8 proteomic profiling by mass spectrometry L1_20220110_MxR_FDR_fourthbatch_DSSOplate_E7_E8.raw 1 19 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220118_MxR_FDR_fourthbatch_DSSOplate_E11_E12_remeasure proteomic profiling by mass spectrometry L1_20220118_MxR_FDR_fourthbatch_DSSOplate_E11_E12_remeasure.raw 1 20 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220118_MxR_FDR_fourthbatch_DSSOplate_E1_E2_remeasure3 proteomic profiling by mass spectrometry L1_20220118_MxR_FDR_fourthbatch_DSSOplate_E1_E2_remeasure3.raw 1 21 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220118_MxR_FDR_fourthbatch_DSSOplate_E3_E4_remeasure2 proteomic profiling by mass spectrometry L1_20220118_MxR_FDR_fourthbatch_DSSOplate_E3_E4_remeasure2.raw 1 22 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220118_MxR_FDR_fourthbatch_DSSOplate_E5_E6_remeasure proteomic profiling by mass spectrometry L1_20220118_MxR_FDR_fourthbatch_DSSOplate_E5_E6_remeasure.raw 1 23 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220118_MxR_FDR_fourthbatch_DSSOplate_E9_E10_remeasure proteomic profiling by mass spectrometry L1_20220118_MxR_FDR_fourthbatch_DSSOplate_E9_E10_remeasure.raw 1 24 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220118_MxR_FDR_fourthbatch_DSSOplate_F11_F12_remeasure proteomic profiling by mass spectrometry L1_20220118_MxR_FDR_fourthbatch_DSSOplate_F11_F12_remeasure.raw 1 25 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220118_MxR_FDR_fourthbatch_DSSOplate_F1_F2_remeasure proteomic profiling by mass spectrometry L1_20220118_MxR_FDR_fourthbatch_DSSOplate_F1_F2_remeasure.raw 1 26 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220118_MxR_FDR_fourthbatch_DSSOplate_F3_F4_remeasure proteomic profiling by mass spectrometry L1_20220118_MxR_FDR_fourthbatch_DSSOplate_F3_F4_remeasure.raw 1 27 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220118_MxR_FDR_fourthbatch_DSSOplate_F5_F6_remeasure proteomic profiling by mass spectrometry L1_20220118_MxR_FDR_fourthbatch_DSSOplate_F5_F6_remeasure.raw 1 28 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220118_MxR_FDR_fourthbatch_DSSOplate_F7_F8_remeasure proteomic profiling by mass spectrometry L1_20220118_MxR_FDR_fourthbatch_DSSOplate_F7_F8_remeasure.raw 1 29 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220118_MxR_FDR_fourthbatch_DSSOplate_F9_F10_remeasure proteomic profiling by mass spectrometry L1_20220118_MxR_FDR_fourthbatch_DSSOplate_F9_F10_remeasure.raw 1 30 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch4 homo sapiens not applicable not applicable not applicable not available not applicable 4 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20220118_MxR_FDR_fourthbatch_DSSOplate_G1_G2_remeasure proteomic profiling by mass spectrometry L1_20220118_MxR_FDR_fourthbatch_DSSOplate_G1_G2_remeasure.raw 1 31 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_B11_B12 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_B11_B12.raw 1 1 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_B1_B2 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_B1_B2.raw 1 2 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_B3_B4 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_B3_B4.raw 1 3 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_B5_B6 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_B5_B6.raw 1 4 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_B7_B8 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_B7_B8.raw 1 5 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_B9_B10 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_B9_B10.raw 1 6 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_C1 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_C1.raw 1 7 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_C10 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_C10.raw 1 8 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_C11 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_C11.raw 1 9 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_C12 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_C12.raw 1 10 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_C2 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_C2.raw 1 11 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_C3 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_C3.raw 1 12 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_C4 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_C4.raw 1 13 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_C5 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_C5.raw 1 14 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_C6 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_C6.raw 1 15 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_C7 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_C7.raw 1 16 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_C8 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_C8.raw 1 17 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_C9 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_C9.raw 1 18 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_D1 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_D1.raw 1 19 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_D10 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_D10.raw 1 20 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_D11 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_D11.raw 1 21 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_D12 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_D12.raw 1 22 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_D2 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_D2.raw 1 23 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_D3 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_D3.raw 1 24 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_D4 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_D4.raw 1 25 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_D5 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_D5.raw 1 26 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_D6 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_D6.raw 1 27 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_D7 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_D7.raw 1 28 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_D8 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_D8.raw 1 29 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_D9 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_D9.raw 1 30 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_E11_E12 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_E11_E12.raw 1 31 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_E1_E2 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_E1_E2.raw 1 32 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_E3_E4 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_E3_E4.raw 1 33 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_E5_E6 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_E5_E6.raw 1 34 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_E7_E8 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_E7_E8.raw 1 35 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_E9_E10 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_E9_E10.raw 1 36 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_F11_F12 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_F11_F12.raw 1 37 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_F1_F2 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_F1_F2.raw 1 38 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_F3_F4 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_F3_F4.raw 1 39 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_F5_F6 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_F5_F6.raw 1 40 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_F7_F8 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_F7_F8.raw 1 41 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_F9_F10 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_F9_F10.raw 1 42 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_G11_G12 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_G11_G12.raw 1 43 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_G1_G2 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_G1_G2.raw 1 44 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_G3_G4 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_G3_G4.raw 1 45 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_G5_G6 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_G5_G6.raw 1 46 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_G7_G8 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_G7_G8.raw 1 47 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 +PXD042173-batch5 homo sapiens not applicable not applicable not applicable not available not applicable 5 synthetic reference enrichment of cross-linked peptides 26.4 Å 30 min room temperature L1_20240412_MxR_FDR_fifthbatch_DSSO_G9_G10 proteomic profiling by mass spectrometry L1_20240412_MxR_FDR_fifthbatch_DSSO_G9_G10.raw 1 48 AC=MS:1002038;NT=label free sample NT=Orbitrap Fusion Lumos;AC=MS:1002732 NT=Data-dependent acquisition;AC=PRIDE:0000449 NT=Trypsin;AC=MS:1001251 NT=Lys-C;AC=MS:1001309 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Oxidation;AC=UNIMOD:35;TA=M;MT=Variable NT=stepped HCD;AC=PRIDE:0000590 stepped 27+-6% 10 ppm 20 ppm NT=strong cation exchange chromatography;AC=PRIDE:0000586 cross-linking mass spectrometry NT=DSSO;AC=XLMOD:02010;CL=yes;TA=K,S,T,Y,nterm;MH=54.01;ML=85.98 strong cation exchange chromatography 0.2-1 mM Tris-HCl NT=dithiothreitol;AC=CHEBI:18320 NT=chloroacetamide;AC=CHEBI:27869 v1.1.0 NT=ms-proteomics;VV=v1.1.0 NT=crosslinking;VV=v1.0.0 NT=human;VV=v1.1.0 diff --git a/psi-document/sdrf-proteomics-specification-v1.1.0-dev.pdf b/psi-document/sdrf-proteomics-specification-v1.1.0-dev.pdf index 644c8f39..bd923d34 100644 Binary files a/psi-document/sdrf-proteomics-specification-v1.1.0-dev.pdf and b/psi-document/sdrf-proteomics-specification-v1.1.0-dev.pdf differ diff --git a/scripts/inject-headers.py b/scripts/inject-headers.py index 5c36fd1a..096c44c3 100755 --- a/scripts/inject-headers.py +++ b/scripts/inject-headers.py @@ -38,11 +38,11 @@ def inject_header(filepath: str, header_html: str, is_dev: bool = False) -> None with open(filepath, 'r', encoding='utf-8') as f: content = f.read() - # Resolve version link based on dev/stable mode + # Resolve version link: only show in dev builds (link back to stable) if is_dev: version_link = 'Stable Version' else: - version_link = 'Dev Version' + version_link = '' resolved_header = header_html.replace(VERSION_LINK_PLACEHOLDER, version_link) # Add has-doc-header class to body @@ -56,17 +56,24 @@ def inject_header(filepath: str, header_html: str, is_dev: bool = False) -> None f.write(content) -def rewrite_version_links(filepath: str, is_dev: bool) -> None: - """Rewrite version links in static HTML pages (index.html, quickstart.html, etc.).""" +def inject_version_link_into_static(filepath: str, is_dev: bool) -> None: + """Inject a 'Stable Version' link into static HTML pages for dev builds.""" + if not is_dev: + return # Stable builds don't show a version link + with open(filepath, 'r', encoding='utf-8') as f: content = f.read() - if is_dev: - content = content.replace( - 'Dev Version', - 'Stable Version' - ) - # No change needed for stable — the source files already have /dev/ links + # Already has a version link — skip + if 'class="version-link"' in content: + return + + # Insert "Stable Version" link before the GitHub link + stable_link = 'Stable Version' + content = content.replace( + ' goes in "optional" section + if ((req === 'optional' && !includedNames[colName]) || + (req === 'recommended' && state.removedColumns.indexOf(colName) !== -1)) { + // Optional not enabled, or recommended that user removed -> "optional" section optionalCols.push(col); } else if (includedNames[colName] && state.extraColumns.indexOf(colName) === -1) { // Included from template (not user-added extra) @@ -734,7 +740,14 @@ item.appendChild(toggleBtn); item.addEventListener('click', function () { - state.addedOptionals.push(col.name); + var req = col.requirement || 'optional'; + if (req === 'recommended') { + // Re-add: remove from removedColumns + var idx = state.removedColumns.indexOf(col.name); + if (idx !== -1) state.removedColumns.splice(idx, 1); + } else { + state.addedOptionals.push(col.name); + } updatePreview(); }); optDiv.appendChild(item); @@ -844,6 +857,11 @@ // Remove from extra var idx = state.extraColumns.indexOf(colName); if (idx !== -1) state.extraColumns.splice(idx, 1); + } else if (col.requirement === 'recommended') { + // Move recommended column to removed list + if (state.removedColumns.indexOf(colName) === -1) { + state.removedColumns.push(colName); + } } else { // Toggle optional template column off (remove from addedOptionals) var idx2 = state.addedOptionals.indexOf(colName); @@ -896,11 +914,16 @@ var columns = resolveColumns(); if (!columns.length) return; - var names = []; + var headers = []; + var values = []; for (var i = 0; i < columns.length; i++) { - names.push(columns[i].name); + headers.push(columns[i].name); + values.push(columns[i].example_value || ''); } - window.open('sdrf-editor.html?columns=' + encodeURIComponent(names.join(','))); + + var tsv = headers.join('\t') + '\n' + values.join('\t') + '\n'; + var encoded = btoa(unescape(encodeURIComponent(tsv))); + window.open('sdrf-editor.html?content=' + encodeURIComponent(encoded)); } /* --------------------------------------------------------------- diff --git a/site/quickstart.html b/site/quickstart.html index 3f89000e..e823eefe 100644 --- a/site/quickstart.html +++ b/site/quickstart.html @@ -109,7 +109,6 @@ Explorer Editor Quick Start - Dev Version GitHub diff --git a/site/sdrf-builder.html b/site/sdrf-builder.html index 86cc604d..f896e2a2 100644 --- a/site/sdrf-builder.html +++ b/site/sdrf-builder.html @@ -17,7 +17,6 @@ Tools Explorer Editor - Dev Version GitHub diff --git a/site/sdrf-data.json b/site/sdrf-data.json index 65c2639a..7bdd4c6f 100644 --- a/site/sdrf-data.json +++ b/site/sdrf-data.json @@ -2,7 +2,7 @@ "statistics": { "total_datasets": 298, "total_samples": 80289, - "generated_at": "2026-03-09T10:31:23.858864Z", + "generated_at": "2026-03-09T20:18:10.203334Z", "organisms": { "Homo sapiens": 51421, "homo sapiens": 10167, @@ -301,9 +301,9 @@ "no cleavage": 5 }, "experiment_types": { - "DDA": 291, + "DDA": 290, "SRM/MRM": 3, - "DIA": 4 + "DIA": 5 }, "label_types": { "Label-free": 229, @@ -329,13 +329,13 @@ "Homo sapiens" ], "diseases": [ - "non-small cell lung cancer", - "small cell lung cancer" + "small cell lung cancer", + "non-small cell lung cancer" ], "instruments": [ "Orbitrap Elite", - "LTQ Orbitrap Velos", - " Orbitrap Fusion Lumos" + " Orbitrap Fusion Lumos", + "LTQ Orbitrap Velos" ], "acquisition_methods": [ "Data-dependent acquisition" @@ -355,8 +355,8 @@ "num_samples": 96, "num_columns": 35, "organisms": [ - "homo sapiens", - "mus musculus" + "mus musculus", + "homo sapiens" ], "diseases": [ "melanoma" @@ -438,12 +438,12 @@ "homo sapiens" ], "diseases": [ - "monoclonal gammopathy", - "multiple myeloma" + "multiple myeloma", + "monoclonal gammopathy" ], "instruments": [ - "Orbitrap Elite", - "LTQ Orbitrap XL" + "LTQ Orbitrap XL", + "Orbitrap Elite" ], "acquisition_methods": [ "Data-dependent acquisition" @@ -516,8 +516,8 @@ "diseases": [ "Rectal Adenocarcinoma", "Rectal Mucinous Adenocarcinoma", - "Colon Mucinous Adenocarcinoma", - "Colon Adenocarcinoma" + "Colon Adenocarcinoma", + "Colon Mucinous Adenocarcinoma" ], "instruments": [ "LTQ Orbitrap Velos" @@ -595,8 +595,8 @@ "Homo sapiens" ], "diseases": [ - "Non-Tumor", - "ovarian high grade serous adenocarcinoma" + "ovarian high grade serous adenocarcinoma", + "Non-Tumor" ], "instruments": [ "Q Exactive" @@ -847,8 +847,8 @@ "Homo sapiens" ], "diseases": [ - "heart infarction", "cardiopulmonary insufficiency", + "heart infarction", "lung embolism" ], "instruments": [ @@ -875,8 +875,8 @@ "Homo sapiens" ], "diseases": [ - "heart infarction", "cardiopulmonary insufficiency", + "heart infarction", "lung embolism" ], "instruments": [ @@ -1868,11 +1868,11 @@ "Homo sapiens" ], "diseases": [ - "Mlignant Melanoma", - "Breast Adenocarcinoma", - "Glioblastoma", + "Pancreatic Carcinoma", "Breast Cancer", - "Pancreatic Carcinoma" + "Breast Adenocarcinoma", + "Mlignant Melanoma", + "Glioblastoma" ], "instruments": [ "LTQ Orbitrap Velos" @@ -1998,16 +1998,16 @@ "Homo sapiens" ], "diseases": [ - "Chronic myelogenous leukemia, BCR-ABL1 positive", - "Prostate carcinoma", - "Lung adenocarcinoma", + "Osteosarcoma", + "Hepatoblastoma", "Human papillomavirus-related endocervical adenocarcinoma", + "Lung adenocarcinoma", + "Prostate carcinoma", "Glioblastoma", - "Hepatoblastoma", + "Chronic myelogenous leukemia, BCR-ABL1 positive", "Childhood T acute lymphoblastic leukemia", - "Colon carcinoma", - "Osteosarcoma", - "Invasive ductal carcinoma, not otherwise specified" + "Invasive ductal carcinoma, not otherwise specified", + "Colon carcinoma" ], "instruments": [ "LTQ Orbitrap Velos" @@ -2180,8 +2180,8 @@ "Homo sapiens" ], "diseases": [ - "Prostate adenocarcinoma", - "control" + "control", + "Prostate adenocarcinoma" ], "instruments": [ "Q Exactive" @@ -2207,8 +2207,8 @@ "Homo sapiens" ], "diseases": [ - "Prostate adenocarcinoma", - "control" + "control", + "Prostate adenocarcinoma" ], "instruments": [ "Q Exactive" @@ -2234,27 +2234,27 @@ "Homo sapiens" ], "diseases": [ + "Anaplastic large cell lymphoma", + "Primary mediastinal large B-cell lymphoma", + "Diffuse large B-cell lymphoma", + "lymphoma", + "Sezary Syndrome", + "Nodular lymphocyte predominant Hodgkin lymphoma", "acute T cell leukemia", "Multiple Myeloma", - "Diffuse large B-cell lymphoma", + "Burkitt's lymphoma", + "Classical Hodgkin lymphoma", "diffuse histiocytic lymphoma", "Mantle cell lymphoma", - "Nodular lymphocyte predominant Hodgkin lymphoma", - "malignant non-Hodgkin's lymphoma", - "Burkitt's lymphoma (American)", + "Aggressive NK-cell leukemia", "Burkitt lymphoma", - "follicular lymphoma", - "Burkitt's lymphoma", - "Anaplastic large cell lymphoma,", - "Primary mediastinal large B-cell lymphoma", - "Classical Hodgkin lymphoma", - "cutaneous T cell lymphoma", - "lymphoma", "Transformed follicular lymphoma", + "Anaplastic large cell lymphoma,", + "follicular lymphoma", + "malignant non-Hodgkin's lymphoma", "plasmacytoma", - "Aggressive NK-cell leukemia", - "Anaplastic large cell lymphoma", - "Sezary Syndrome" + "Burkitt's lymphoma (American)", + "cutaneous T cell lymphoma" ], "instruments": [ "LTQ Orbitrap XL" @@ -2280,8 +2280,8 @@ "Homo sapiens" ], "diseases": [ - "Prostate adenocarcinoma", - "control" + "control", + "Prostate adenocarcinoma" ], "instruments": [ "Q Exactive" @@ -2307,8 +2307,8 @@ "Homo sapiens" ], "diseases": [ - "high grade serous ovarian cancer", - "epithelial ovarian cancer" + "epithelial ovarian cancer", + "high grade serous ovarian cancer" ], "instruments": [ "Q Exactive HF-X", @@ -2336,36 +2336,36 @@ ], "diseases": [ "clear cell renal carcinoma", - "malignant melanoma", - "promyelocytic leukemia", - "melanoma", - "ovarian adenocarcinoma", - "skin cutaneous melanoma", - "renal clear cell adenocarcinoma", - "renal carcinoma", - "acute lymphoblastic leukemia", - "glioblastoma", - "breast ductal adenocarcinoma", + "colon carcinoma", + "acute T lymphoblastic leukaemia", + "prostate adenocarcinoma", + "breast carcinoma", + "renal adenocarcinoma", + "ovarian serous cystadenocarcinoma", + "renal cell adenocarcinoma", "large cell lung carcinoma", + "renal carcinoma", "astrocytoma", + "non-small cell lung carcinoma", + "glioma", + "malignant melanoma", "chronic myelogenic leukemia", - "ovarian serous cystadenocarcinoma", - "renal cell carcinoma", - "breast adenocarcinoma", - "renal adenocarcinoma", "mesothelioma", - "non-small cell lung carcinoma", - "renal cell adenocarcinoma", "ovarian carcinoma", - "prostate adenocarcinoma", - "acute T lymphoblastic leukaemia", + "breast adenocarcinoma", + "acute lymphoblastic leukemia", + "immunoblastic large cell lymphoma", + "promyelocytic leukemia", "lung carcinoma", - "breast carcinoma", + "colon adenocarcinoma", + "renal cell carcinoma", + "ovarian adenocarcinoma", "plasmacytoma", - "colon carcinoma", - "immunoblastic large cell lymphoma", - "glioma", - "colon adenocarcinoma" + "glioblastoma", + "breast ductal adenocarcinoma", + "melanoma", + "renal clear cell adenocarcinoma", + "skin cutaneous melanoma" ], "instruments": [ "TripleTOF 5600" @@ -2417,8 +2417,8 @@ "Homo sapiens" ], "diseases": [ - "Prostate adenocarcinoma", - "control" + "control", + "Prostate adenocarcinoma" ], "instruments": [ "Q Exactive" @@ -2496,8 +2496,8 @@ "Homo sapiens" ], "diseases": [ - "Prostate adenocarcinoma", - "control" + "control", + "Prostate adenocarcinoma" ], "instruments": [ "Q Exactive" @@ -2573,8 +2573,8 @@ "Homo sapiens" ], "diseases": [ - "uninfected", - "obesity" + "obesity", + "uninfected" ], "instruments": [ "Q Exactive HF" @@ -2651,11 +2651,11 @@ "Homo sapiens" ], "diseases": [ - "neuroblastoma", + "lung adenocarcinoma", + "breast cancer", "colon cancer", "epithelial cervix carcinoma", - "breast cancer", - "lung adenocarcinoma" + "neuroblastoma" ], "instruments": [ "Q Exactive" @@ -2925,8 +2925,8 @@ "Homo sapiens" ], "diseases": [ - "control", - "squamous cell carcinoma" + "squamous cell carcinoma", + "control" ], "instruments": [ "Q Exactive Plus" @@ -2978,8 +2978,8 @@ "Homo sapiens" ], "diseases": [ - "control", - "squamous cell carcinoma" + "squamous cell carcinoma", + "control" ], "instruments": [ "Q Exactive Plus" @@ -3205,8 +3205,8 @@ "Homo sapiens" ], "diseases": [ - "Normal Colorectum", - "Colorectal cancer" + "Colorectal cancer", + "Normal Colorectum" ], "instruments": [ "LTQ Orbitrap Velos" @@ -3612,8 +3612,8 @@ "Homo sapiens" ], "diseases": [ - "malignant melanoma", - "amelanotic melanoma" + "amelanotic melanoma", + "malignant melanoma" ], "instruments": [ " Orbitrap Elite" @@ -3714,15 +3714,15 @@ "homo sapiens" ], "diseases": [ - "non-small cell lung adenocarcinoma", - "amelanotic melanoma", - "astrocytoma", + "colon adenocarcinoma", "renal cell carcinoma", - "ovarian serous cystadenocarcinoma", + "astrocytoma", + "invasive ductal carcinoma", "childhood T acute lymphoblastic leukemia", - "colon adenocarcinoma", - "prostate carcinoma", - "invasive ductal carcinoma" + "ovarian serous cystadenocarcinoma", + "amelanotic melanoma", + "non-small cell lung adenocarcinoma", + "prostate carcinoma" ], "instruments": [ "Orbitrap Elite" @@ -3748,34 +3748,34 @@ "homo sapiens" ], "diseases": [ - "non-small cell lung adenocarcinoma", "clear cell renal carcinoma", - "adult T acute lymphoblastic leukemia", - "amelanotic melanoma", - "high grade ovarian serous adenocarcinoma", - "melanoma", - "metastatic melanoma", - "Ovarian Endometrioid Adenocarcinoma", - "adult acute myeloid leukemia", - "brain glioblastoma", - "astrocytoma", - "Colon adenocarcinoma", - "renal cell carcinoma", + "colon carcinoma", + "childhood T acute lymphoblastic leukemia", "ovarian serous cystadenocarcinoma", - "lung adenocarcinoma", - "breast adenocarcinoma", "Pleural Epithelioid Mesothelioma", - "non-small cell lung carcinoma", - "anaplastic large cell lymphoma", "chronic myelogenous leukemia", + "cutaneous melanoma", + "lung adenocarcinoma", + "anaplastic large cell lymphoma", + "astrocytoma", + "non-small cell lung carcinoma", + "Colon adenocarcinoma", + "breast adenocarcinoma", + "Ovarian Endometrioid Adenocarcinoma", + "high grade ovarian serous adenocarcinoma", "multiple myeloma", - "childhood T acute lymphoblastic leukemia", "invasive ductal carcinoma", + "non-small cell lung adenocarcinoma", "gliosarcoma", - "colon carcinoma", "colon adenocarcinoma", - "cutaneous melanoma", - "prostate carcinoma" + "renal cell carcinoma", + "brain glioblastoma", + "melanoma", + "amelanotic melanoma", + "metastatic melanoma", + "prostate carcinoma", + "adult T acute lymphoblastic leukemia", + "adult acute myeloid leukemia" ], "instruments": [ "LTQ Orbitrap XL ETD" @@ -3801,34 +3801,34 @@ "homo sapiens" ], "diseases": [ - "non-small cell lung adenocarcinoma", "clear cell renal carcinoma", - "adult T acute lymphoblastic leukemia", - "amelanotic melanoma", - "high grade ovarian serous adenocarcinoma", - "melanoma", - "metastatic melanoma", - "Ovarian Endometrioid Adenocarcinoma", - "adult acute myeloid leukemia", - "brain glioblastoma", - "astrocytoma", - "renal cell carcinoma", + "colon carcinoma", + "childhood T acute lymphoblastic leukemia", "ovarian serous cystadenocarcinoma", - "lung adenocarcinoma", - "breast adenocarcinoma", "Pleural Epithelioid Mesothelioma", + "chronic myelogenous leukemia", + "cutaneous melanoma", + "lung adenocarcinoma", "anaplastic large cell lymphoma", + "astrocytoma", "non-small cell lung carcinoma", - "chronic myelogenous leukemia", + "breast adenocarcinoma", + "Ovarian Endometrioid Adenocarcinoma", + "high grade ovarian serous adenocarcinoma", "multiple myeloma", - "childhood T acute lymphoblastic leukemia", - "rectosigmoid adenocarcinoma", "invasive ductal carcinoma", + "non-small cell lung adenocarcinoma", "gliosarcoma", - "colon carcinoma", "colon adenocarcinoma", - "cutaneous melanoma", - "prostate carcinoma" + "renal cell carcinoma", + "brain glioblastoma", + "melanoma", + "amelanotic melanoma", + "metastatic melanoma", + "prostate carcinoma", + "adult T acute lymphoblastic leukemia", + "rectosigmoid adenocarcinoma", + "adult acute myeloid leukemia" ], "instruments": [ "LTQ Orbitrap XL ETD" @@ -3953,8 +3953,8 @@ "num_samples": 28, "num_columns": 32, "organisms": [ - "homo sapiens", - "mus musculus" + "mus musculus", + "homo sapiens" ], "diseases": [], "instruments": [ @@ -4164,8 +4164,8 @@ ], "diseases": [ "recessive dystrophyic epidermolysis bullosa cutaneous squamous cell carcinoma", - "Low-risk cutaneous squamous cell carcinoma", - "metastasizing cutaneous squamous cell carcinoma" + "metastasizing cutaneous squamous cell carcinoma", + "Low-risk cutaneous squamous cell carcinoma" ], "instruments": [ "Q Exactive Plus" @@ -4217,42 +4217,42 @@ "homo sapiens" ], "diseases": [ - "Alzheimer's disease/Parkinson's disease5", + "Parkinson's disease7", "control6", - "Alzheimer's disease 8", - "Parkinson's disease5", + "Alzheimer's disease/Parkinson's disease0", "Alzheimer's disease 3", - "Parkinson's disease6", - "control7", - "Alzheimer's disease/Parkinson's disease9", - "Alzheimer's disease/Parkinson's disease7", + "Parkinson's disease4", "Alzheimer's disease 7", - "Alzheimer's disease 6", - "Parkinson's disease7", - "control8", - "Parkinson's disease3", - "Alzheimer's disease0", - "Alzheimer's disease/Parkinson's disease3", - "control0", - "Parkinson's disease9", + "Alzheimer's disease", + "Alzheimer's disease 8", "Parkinson's disease0", - "Alzheimer's disease/Parkinson's disease", "Alzheimer's disease 9", + "Parkinson's disease5", + "control4", "control", - "Alzheimer's disease/Parkinson's disease0", - "Alzheimer's disease", - "Parkinson's disease", - "control3", - "Alzheimer's disease/Parkinson's disease4", - "Alzheimer's disease/Parkinson's disease8", "Parkinson's disease8", - "control4", - "Alzheimer's disease/Parkinson's disease6", - "Alzheimer's disease 5", - "control5", "Alzheimer's disease 4", + "Parkinson's disease6", "control9", - "Parkinson's disease4" + "Alzheimer's disease/Parkinson's disease7", + "Alzheimer's disease/Parkinson's disease8", + "control0", + "control5", + "Alzheimer's disease0", + "Alzheimer's disease/Parkinson's disease6", + "Parkinson's disease", + "Alzheimer's disease/Parkinson's disease4", + "Parkinson's disease3", + "control7", + "Alzheimer's disease/Parkinson's disease3", + "Alzheimer's disease/Parkinson's disease", + "Alzheimer's disease 5", + "Alzheimer's disease/Parkinson's disease9", + "Alzheimer's disease 6", + "Alzheimer's disease/Parkinson's disease5", + "control8", + "Parkinson's disease9", + "control3" ], "instruments": [ "Orbitrap Fusion" @@ -4380,13 +4380,13 @@ "Homo sapiens" ], "diseases": [ + "medulallary carcinoma", "primary breast tumor", + "primary ductal carcinoma", + "metastatic carcinoma", "breast carcinoma", "ductal carcinoma", "fibrocystic disease", - "medulallary carcinoma", - "metastatic carcinoma", - "primary ductal carcinoma", "primary acantholytic squamous cell carcinoma" ], "instruments": [ @@ -4539,12 +4539,12 @@ "Homo Sapiens" ], "diseases": [ - "basal-like breast carcinoma", "Normal Breast-Like Subtype of Breast Carcinoma", "breast cancer", "luminal A breast carcinoma", + "luminal B breast carcinoma", "HER2 Positive Breast Carcinoma", - "luminal B breast carcinoma" + "basal-like breast carcinoma" ], "instruments": [ "Q Exactive" @@ -4594,10 +4594,10 @@ "Homo sapiens" ], "diseases": [ - "compensated hypertrophy", - "cardiac hypertrophy", "ischemic cardiomyopathy", - "dilated cardiomyopathy" + "compensated hypertrophy", + "dilated cardiomyopathy", + "cardiac hypertrophy" ], "instruments": [ "Q Exactive" @@ -4772,13 +4772,13 @@ "Homo sapiens" ], "diseases": [ + "other", + "mucosal", + "acral lentiginous melanoma", "unknown", "superficial spreading melanoma", "lentigo maligna melanoma", - "other", - "nodular melanoma", - "mucosal", - "acral lentiginous melanoma" + "nodular melanoma" ], "instruments": [ "Q Exactive Plus", @@ -4854,9 +4854,9 @@ ], "diseases": [], "instruments": [ + "Orbitrap Fusion Lumos", "Q Exactive HF", - "Q Exactive Plus", - "Orbitrap Fusion Lumos" + "Q Exactive Plus" ], "acquisition_methods": [ "Data-dependent acquisition" @@ -4932,14 +4932,14 @@ "Homo sapiens" ], "diseases": [ - "'Crohn''s Disease Irritable Bowel Syndrome'", - "Healthy control", + "Ulcerative Colitis active stage", "Ulcerative Colitis remission stage", - "Irritable Bowel Syndrome", + "'Crohn''s Disease Irritable Bowel Syndrome'", "Colon Adenoma", - "Gastric Carcinoma", "'Crohn''s Disease'", - "Ulcerative Colitis active stage" + "Healthy control", + "Irritable Bowel Syndrome", + "Gastric Carcinoma" ], "instruments": [ "Q Exactive HF" @@ -5269,8 +5269,8 @@ "homo sapiens" ], "diseases": [ - "non-alcoholic fatty liver disease", "cirrhosis of liver", + "non-alcoholic fatty liver disease", "type II diabetes mellitus" ], "instruments": [ @@ -5372,8 +5372,8 @@ "Homo sapiens" ], "diseases": [ - "Non-demented control", "Alzheimer's diseasemedial", + "Non-demented control", "Alzheimer's disease" ], "instruments": [ @@ -5551,8 +5551,8 @@ ], "diseases": [], "instruments": [ - "Q Exactive Plus", - "TSQ Quantiva" + "TSQ Quantiva", + "Q Exactive Plus" ], "acquisition_methods": [ "selected reaction monitoring" @@ -5929,9 +5929,9 @@ ], "diseases": [ "gastric carcinoma", + "Hepatoblastoma", "epithelial cervix carcinoma", - "Colorectal cancer", - "Hepatoblastoma" + "Colorectal cancer" ], "instruments": [ "Orbitrap Fusion Lumos" @@ -5983,8 +5983,8 @@ "Homo sapiens" ], "diseases": [ - "breast cancer", - "nontumorigenic" + "nontumorigenic", + "breast cancer" ], "instruments": [ "LTQ" @@ -6212,8 +6212,8 @@ "Homo sapiens" ], "diseases": [ - "non-small cell lung cancer", - "small cell lung cancer" + "small cell lung cancer", + "non-small cell lung cancer" ], "instruments": [ " Orbitrap Fusion Lumos" @@ -6286,9 +6286,9 @@ "num_samples": 360, "num_columns": 32, "organisms": [ - "plasmodium falciparum", + "plasmodium knowlesi", "Homo sapiens", - "plasmodium knowlesi" + "plasmodium falciparum" ], "diseases": [], "instruments": [ @@ -6362,8 +6362,8 @@ "num_samples": 160, "num_columns": 31, "organisms": [ - "plasmodium falciparum", - "plasmodium knowlesi" + "plasmodium knowlesi", + "plasmodium falciparum" ], "diseases": [], "instruments": [ @@ -6387,8 +6387,8 @@ "num_samples": 160, "num_columns": 31, "organisms": [ - "plasmodium falciparum", - "plasmodium knowlesi" + "plasmodium knowlesi", + "plasmodium falciparum" ], "diseases": [], "instruments": [ @@ -6412,8 +6412,8 @@ "num_samples": 160, "num_columns": 31, "organisms": [ - "plasmodium falciparum", - "plasmodium knowlesi" + "plasmodium knowlesi", + "plasmodium falciparum" ], "diseases": [], "instruments": [ @@ -6542,8 +6542,8 @@ ], "diseases": [], "instruments": [ - "Q Exactive Plus", - "Orbitrap Velos Pro" + "Orbitrap Velos Pro", + "Q Exactive Plus" ], "acquisition_methods": [ "Data-dependent acquisition" @@ -6613,14 +6613,14 @@ "num_samples": 720, "num_columns": 30, "organisms": [ - "Bifidobacterium longum", - "Bacteroides thetaiotaomicron", + "Blautia producta", "Clostridium butyricum", - "lactobacillus", "Anaerostipes caccae", "Escherichia coli K-12", - "Blautia producta", - "clostridium" + "lactobacillus", + "Bifidobacterium longum", + "clostridium", + "Bacteroides thetaiotaomicron" ], "diseases": [], "instruments": [ @@ -6676,8 +6676,8 @@ "chronic myelogenous leukemia" ], "instruments": [ - "Q Exactive Plus", - "Orbitrap Fusion Lumos" + "Orbitrap Fusion Lumos", + "Q Exactive Plus" ], "acquisition_methods": [ "Data-dependent acquisition" @@ -6753,8 +6753,8 @@ ], "diseases": [], "instruments": [ - "Q Exactive Plus", - "Orbitrap Fusion Lumos" + "Orbitrap Fusion Lumos", + "Q Exactive Plus" ], "acquisition_methods": [ "Data-dependent acquisition" @@ -6777,8 +6777,8 @@ "Homo sapiens" ], "diseases": [ - "adenocarcinoma", - "colon cancer" + "colon cancer", + "adenocarcinoma" ], "instruments": [ "Q Exactive HF" @@ -7088,10 +7088,10 @@ "Orbitrap Exploris 480" ], "acquisition_methods": [ - "Data-dependent acquisition", - "Data-independent acquisition" + "Data-independent acquisition", + "Data-dependent acquisition" ], - "experiment_type": "DDA", + "experiment_type": "DIA", "label_type": "Label-free", "template": "unknown", "version": "unknown" @@ -7233,16 +7233,16 @@ "homo sapiens" ], "diseases": [ + "native_5", + "decellularized_4", "decellularized_2", "native_3", - "decellularized_4", - "native_5", - "decellularized_3", - "decellularized_1", "native_2", "native_1", + "decellularized_3", "decellularized_5", - "native_4" + "native_4", + "decellularized_1" ], "instruments": [ "LTQ" @@ -7393,8 +7393,8 @@ ], "diseases": [], "instruments": [ - "SCIEX instrument model", - "TripleTOF 5600+" + "TripleTOF 5600+", + "SCIEX instrument model" ], "acquisition_methods": [ "data-dependent acquisition" @@ -8072,8 +8072,8 @@ ], "diseases": [], "instruments": [ - "Q Exactive HF-X", - "timsTOF Pro 2" + "timsTOF Pro 2", + "Q Exactive HF-X" ], "acquisition_methods": [ "data-dependent acquisition" diff --git a/site/sdrf-explorer.html b/site/sdrf-explorer.html index 8c08fe4c..95df8914 100644 --- a/site/sdrf-explorer.html +++ b/site/sdrf-explorer.html @@ -218,7 +218,6 @@ Explorer Editor Contributors - Dev Version GitHub diff --git a/site/sdrf-terms.html b/site/sdrf-terms.html index 206ceded..ce1db14c 100644 --- a/site/sdrf-terms.html +++ b/site/sdrf-terms.html @@ -366,7 +366,6 @@ Explorer Editor Contributors - Dev Version GitHub