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Description
Description of the bug
Running the pipeline on "real" data returns an error, with the log showing the cause as a missing file (test.fa). Creating this file in the current/working directory (e.g. touch /scratch/tblimkie/Testing_bacpaq/test.fa) resolves the error, allowing the pipeline to proceed.
Command used and terminal output
#!/bin/bash
# Load modules on cedar, after moving to an interactive node via `salloc`
module purge && module load StdEnv/2023 nextflow/24.04.4 apptainer/1.2.4
nextflow -log Logs/nextflow_test_run.log run cidgoh/bacpaq \
-r v1.0.1 \
-profile cedar,singularity \
--account df-ahylee \
--input sample_sheet_test.csv \
--outdir Nextflow_results_test \
--genome_size 5700000 \
--depth_cut_off 40 \
--trim_tool trimmomatic \
--skip_kraken2 true \
--confindr_db /home/tblimkie/.confindr_db \
--skip_taxonomy_qc true \
--skip_dehosting true \
--sr_assembler spades \
--skip_porechop true \
--skip_subsampling true \
--skip_pycoqc true \
--skip_checkm true \
--skip_busco true \
--skip_gene_annotation true \
--skip_prokka true \
--skip_bakta true \
--skip_abricate true \
--skip_amr_annotation true \
--skip_rgi true \
--skip_abritamr true \
--skip_amrfinderplus true \
--skip_phage_annotation true \
--skip_mobsuite true \
--skip_plasmidfinder true \
--skip_crispr true \
--skip_roary true \
--skip_pirate trueRelevant files
nextflow_test_run.log
sample_sheet_test.csv
System information
Nextflow: 24.04.4
Hardware: Cedar
Executor: Local (salloc)
Container: Singularity
OS: Linux
Version of bacpaq: v1.0.1
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bugSomething isn't workingSomething isn't working