Error in spatialCluster

[littlecabiria]

Hi Team, just a quick question that it seems like there are some errors when I was running the spatialCluster() function. I supposed that there are some errors in my sce object but I still tried to find it. Could someone help me please?

>   sim_sce <- spatialPreprocess(
+     sim_sce,
+     platform = plat,
+     n.PCs = 7,
+     n.HVGs = 150,
+     skip.PCA = FALSE,
+     log.normalize = TRUE,
+     assay.type = "logcounts"
+   )
Warning message:
In regularize.values(x, y, ties, missing(ties), na.rm = na.rm) :
  collapsing to unique 'x' values
>   single_sim_SRT <- BayesSpace::spatialCluster(sce=sim_sce, 
+                                    q=length(unique(real_sce@colData$spatial.cluster)), 
+                                    d=7, 
+                                    platform=plat, 
+                                    nrep=50000, 
+                                    gamma=2)
Neighbors were identified for 0 out of 278 spots.
Fitting model...
Error: Not compatible with requested type: [type=NULL; target=double].

false_sce.rds.zip

[edward130603]

your simulated PCA matrix has a singular covariance matrix. probably need to change up your data generating steps to make it more realistic.

side note, BayesSpace isn't finding neighbors on your data here, so it won't end up doing any spatial smoothing. you will need to edit the code to detect neighbors.