11---
22layout : tutorial_hands_on
3-
4- title : " Assembly of metagenomic sequencing data"
5- zenodo_link : " https://zenodo.org/record/7818827"
3+ title : Assembly of metagenomic sequencing data
4+ zenodo_link : https://zenodo.org/record/7818827
65level : Introductory
76questions :
8- - " Why metagenomic data should be assembled?"
9- - " What is the difference between co-assembly and individual assembly?"
10- - " What is the difference between reads, contigs and scaffolds?"
11- - " How tools based on De Bruijn graph work?"
12- - " How to assess the quality of metagenomic data assembly?"
7+ - Why metagenomic data should be assembled?
8+ - What is the difference between co-assembly and individual assembly?
9+ - What is the difference between reads, contigs and scaffolds?
10+ - How tools based on De Bruijn graph work?
11+ - How to assess the quality of metagenomic data assembly?
1312objectives :
14- - " Describe what an assembly is."
15- - " Explain the difference between co-assembly and individual assembly."
16- - " Explain the difference between reads, contigs and scaffolds."
17- - " Explain how tools based on de Bruijn graph work."
18- - " Evaluate the quality of the Assembly with QUAST, Bowtie2, and CoverM-Contig."
19- - " Construct and apply simple assembly pipelines on short read data."
20- time_estimation : " 2H "
13+ - Describe what an assembly is.
14+ - Explain the difference between co-assembly and individual assembly.
15+ - Explain the difference between reads, contigs and scaffolds.
16+ - Explain how tools based on de Bruijn graph work.
17+ - Evaluate the quality of the Assembly with QUAST, Bowtie2, and CoverM-Contig.
18+ - Construct and apply simple assembly pipelines on short read data.
19+ time_estimation : 2H
2120key_points :
22- - " Assembly groups reads into contigs and scaffolds."
23- - " de Brujin Graphs use k-mers to assembly reads."
24- - " MetaSPAdes and MEGAHIT are short-read assemblers."
25- - " MetaQUAST is a tool to assess metagenomic assembly quality."
21+ - Assembly groups reads into contigs and scaffolds.
22+ - de Brujin Graphs use k-mers to assembly reads.
23+ - MetaSPAdes and MEGAHIT are short-read assemblers.
24+ - MetaQUAST is a tool to assess metagenomic assembly quality.
2625edam_ontology :
27- - topic_3174 # Metagenomics
28- - topic_0196 # Sequence assembly
26+ - topic_3174
27+ - topic_0196
2928contributions :
3029 authorship :
31- - plushz
32- - bebatut
33- - vinisalazar
34- - paulzierep
30+ - plushz
31+ - bebatut
32+ - vinisalazar
33+ - paulzierep
3534 funding :
36- - gallantries
35+ - gallantries
3736subtopic : metagenomics
3837tags :
39- - assembly
40- - metagenomics
41- - microgalaxy
38+ - assembly
39+ - metagenomics
40+ - microgalaxy
4241redirect_from :
43- - /topics/metagenomics/tutorials/metagenomics-assembly/tutorial
44-
42+ - " /topics/metagenomics/tutorials/metagenomics-assembly/tutorial"
4543recordings :
4644- youtube_id : 1ZYGy85Im7w
4745 date : ' 2023-05-02'
@@ -52,9 +50,19 @@ recordings:
5250 captioners :
5351 - bebatut
5452 - sophia120199
53+ - youtube_id : KzqLR4btsic
54+ length : 1H15M
55+ galaxy_version : 25.1.2.dev0
56+ date : ' 2026-03-26'
57+ speakers :
58+ - gdefazio
59+ captioners :
60+ - gdefazio
61+ - natalie-wa
5562
5663---
5764
65+
5866Metagenomics involves the extraction, sequencing and analysis of combined genomic DNA from ** entire microbiome** samples. It includes then DNA from ** many different organisms** , with different taxonomic background.
5967
6068Reconstructing the genomes of microorganisms in the sampled communities is critical step in analyzing metagenomic data. To do that, we can use ** assembly** and assemblers, * i.e.* computational programs that stich together the small fragments of sequenced DNA produced by sequencing instruments.
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