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Merge pull request #6748 from galaxyproject/google-form/recordings-1774609381
[Google Form] New Recording Submission! Metagenomic Assembly, Giuseppe
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topics/microbiome/tutorials/metagenomics-assembly/tutorial.md

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---
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layout: tutorial_hands_on
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title: "Assembly of metagenomic sequencing data"
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zenodo_link: "https://zenodo.org/record/7818827"
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title: Assembly of metagenomic sequencing data
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zenodo_link: https://zenodo.org/record/7818827
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level: Introductory
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questions:
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- "Why metagenomic data should be assembled?"
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- "What is the difference between co-assembly and individual assembly?"
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- "What is the difference between reads, contigs and scaffolds?"
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- "How tools based on De Bruijn graph work?"
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- "How to assess the quality of metagenomic data assembly?"
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- Why metagenomic data should be assembled?
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- What is the difference between co-assembly and individual assembly?
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- What is the difference between reads, contigs and scaffolds?
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- How tools based on De Bruijn graph work?
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- How to assess the quality of metagenomic data assembly?
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objectives:
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- "Describe what an assembly is."
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- "Explain the difference between co-assembly and individual assembly."
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- "Explain the difference between reads, contigs and scaffolds."
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- "Explain how tools based on de Bruijn graph work."
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- "Evaluate the quality of the Assembly with QUAST, Bowtie2, and CoverM-Contig."
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- "Construct and apply simple assembly pipelines on short read data."
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time_estimation: "2H"
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- Describe what an assembly is.
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- Explain the difference between co-assembly and individual assembly.
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- Explain the difference between reads, contigs and scaffolds.
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- Explain how tools based on de Bruijn graph work.
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- Evaluate the quality of the Assembly with QUAST, Bowtie2, and CoverM-Contig.
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- Construct and apply simple assembly pipelines on short read data.
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time_estimation: 2H
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key_points:
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- "Assembly groups reads into contigs and scaffolds."
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- "de Brujin Graphs use k-mers to assembly reads."
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- "MetaSPAdes and MEGAHIT are short-read assemblers."
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- "MetaQUAST is a tool to assess metagenomic assembly quality."
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- Assembly groups reads into contigs and scaffolds.
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- de Brujin Graphs use k-mers to assembly reads.
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- MetaSPAdes and MEGAHIT are short-read assemblers.
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- MetaQUAST is a tool to assess metagenomic assembly quality.
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edam_ontology:
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- topic_3174 # Metagenomics
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- topic_0196 # Sequence assembly
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- topic_3174
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- topic_0196
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contributions:
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authorship:
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- plushz
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- bebatut
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- vinisalazar
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- paulzierep
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- plushz
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- bebatut
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- vinisalazar
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- paulzierep
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funding:
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- gallantries
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- gallantries
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subtopic: metagenomics
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tags:
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- assembly
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- metagenomics
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- microgalaxy
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- assembly
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- metagenomics
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- microgalaxy
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redirect_from:
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- /topics/metagenomics/tutorials/metagenomics-assembly/tutorial
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- "/topics/metagenomics/tutorials/metagenomics-assembly/tutorial"
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recordings:
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- youtube_id: 1ZYGy85Im7w
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date: '2023-05-02'
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captioners:
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- bebatut
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- sophia120199
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- youtube_id: KzqLR4btsic
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length: 1H15M
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galaxy_version: 25.1.2.dev0
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date: '2026-03-26'
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speakers:
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- gdefazio
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captioners:
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- gdefazio
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- natalie-wa
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---
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Metagenomics involves the extraction, sequencing and analysis of combined genomic DNA from **entire microbiome** samples. It includes then DNA from **many different organisms**, with different taxonomic background.
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Reconstructing the genomes of microorganisms in the sampled communities is critical step in analyzing metagenomic data. To do that, we can use **assembly** and assemblers, *i.e.* computational programs that stich together the small fragments of sequenced DNA produced by sequencing instruments.

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