beta-carotene 3-hydroxylase (EC:1.14.15.24)

[sjm41]

There is currently no GO term for:

EC:1.14.15.24
name: beta-carotene 3-hydroxylase
alt_name: beta-carotene 3,3'-monooxygenase
reaction: all-trans-beta-carotene + 4 H(+) + 2 O2 + 4 reduced [2Fe-2S]-[ferredoxin] = all-trans-zeaxanthin + 2 H2O + 4 oxidized [2Fe-2S]-[ferredoxin]
= RHEA:30331 = MetaCyc:RXN1F-152

However, at least six GO terms reference this activity in some way:

---

id: GO:0052608
name: echinenone 3-hydroxylase activity (no annotations)
def: "Catalysis of the reaction: echinenone + a reduced electron acceptor + oxygen = 3-hydroxyechinenone + an oxidized electron acceptor + H2O." [MetaCyc:RXN-8214]
synonym: "beta-carotene hydroxylase activity" BROAD []
synonym: "beta-ring carotenoid 3-hydroxylase activity" BROAD [MetaCyc:MONOMER-12386]
synonym: "carotene beta-ring hydroxylase activity" BROAD []
xref: MetaCyc:RXN-8214
is_a: GO:0004497 ! monooxygenase activity

=> MetaCyc:RXN-8214 is unofficial reaction of EC:1.14.15.24/MetaCyc:RXN1F-152

---

id: GO:0052610
name: beta-cryptoxanthin hydroxylase activity (0 EXP annotations)
def: "Catalysis of the reaction: beta-cryptoxanthin + a reduced electron acceptor + oxygen = zeaxanthin + an oxidized electron acceptor + H2O." [RHEA:30327]
synonym: "beta-carotene hydroxylase activity" BROAD []
synonym: "beta-ring carotenoid 3-hydroxylase activity" BROAD [MetaCyc:MONOMER-12386]
synonym: "carotene beta-ring hydroxylase activity" BROAD []
xref: MetaCyc:RXN-8026
xref: RHEA:30327
is_a: GO:0004497 ! monooxygenase activity

=> RHEA:30327 is part of RHEA:30331 (= EC:1.14.15.24)
=> MetaCyc:RXN-8026 is stated to be subreaction of EC:1.14.15.24

---

id: GO:0052611 (0 EXP annotations)
name: beta-carotene 3-hydroxylase activity
def: "Catalysis of the reaction: beta-carotene + a reduced electron acceptor + oxygen = beta-cryptoxanthin + an oxidized electron acceptor + H2O." [RHEA:30323]
synonym: "beta-carotene hydroxylase activity" BROAD []
synonym: "carotene beta-ring hydroxylase activity" BROAD []
xref: MetaCyc:RXN-8025
xref: RHEA:30323
is_a: GO:0004497 ! monooxygenase activity
property_value: term_tracker_item "#5604" xsd:anyURI

=> RHEA:30323 is part of RHEA:30331 (= EC 1.14.15.24)
=> MetaCyc:RXN-8025 is stated to be subreaction of EC:1.14.15.24

---

id: GO:0052612 (no annotations)
name: adonirubin 3-hydroxylase activity
def: "Catalysis of the reaction: adonirubin + a reduced electron acceptor + oxygen = 3S,3'S-astaxanthin + an oxidized electron acceptor + H2O." [MetaCyc:RXN-8187]
synonym: "beta-carotene hydroxylase activity" BROAD []
synonym: "beta-ring carotenoid 3-hydroxylase activity" BROAD [MetaCyc:MONOMER-12386]
synonym: "carotene beta-ring hydroxylase activity" BROAD []
xref: MetaCyc:RXN-8187
is_a: GO:0004497 ! monooxygenase activity

=> MetaCyc:RXN-8187 is stated to be subreaction of EC:1.14.15.24

---

id: GO:0052613 (no annotations)
name: canthaxanthin 3-hydroxylase activity
def: "Catalysis of the reaction: canthaxanthin + a reduced electron acceptor + oxygen = adonirubin + an oxidized electron acceptor + H2O." [MetaCyc:RXN-8186]
synonym: "beta-carotene hydroxylase activity" BROAD []
synonym: "beta-ring carotenoid 3-hydroxylase activity" BROAD [MetaCyc:MONOMER-12386]
synonym: "carotene beta-ring hydroxylase activity" BROAD []
xref: MetaCyc:RXN-8186
is_a: GO:0004497 ! monooxygenase activity

=> MetaCyc:RXN-8186 is stated to be subreaction of EC:1.14.15.24

---

id: GO:0010291 (1 EXP annotation, to Arabidopsis CYP97A3 from PMID:16492736)
name: carotene beta-ring hydroxylase activity
def: "Catalysis of the reaction: a carotene + a reduced electron acceptor + O2 = C3-hydroxylated carotene + an oxidized electron acceptor + H2O. This is a general reaction to represent the C3 hydroxylation of the beta ring of a carotene." [MetaCyc:MONOMER-12386, PMID:16492736]
synonym: "beta-carotene hydroxylase activity" NARROW []
is_a: GO:0004497 ! monooxygenase activity
property_value: term_tracker_item "#5604" xsd:anyURI

=> this sounds like the same activity as EC:1.14.15.24. Would you agree @tberardini ?

---

So, seems like all 6 of these GO terms sh/could be merged into a new single GO term representing EC:1.14.15.24/RHEA:30331/MetaCyc:RXN1F-152.