diff --git a/onnxruntime/core/providers/openvino/backend_utils.cc b/onnxruntime/core/providers/openvino/backend_utils.cc
index 73fbe9a0fa76f..7027861f0c4dc 100644
--- a/onnxruntime/core/providers/openvino/backend_utils.cc
+++ b/onnxruntime/core/providers/openvino/backend_utils.cc
@@ -150,6 +150,11 @@ CreateOVModel(std::string&& model,
       LOGS_DEFAULT(INFO) << log_tag << "Reshaping the ov tensor to specified shape";
       ov_model->reshape(session_context.reshape);
     }
+
+    if (!session_context.layout.empty()) {
+      LOGS_DEFAULT(INFO) << log_tag << "Setting the ov tensor layout to specified layout";
+      ov_model = Set_Layout(ov_model, session_context.layout);
+    }
     // Check for Constant Folding
     if ((session_context.device_type != "NPU") && !session_context.is_wholly_supported_graph) {
       ov::pass::ConstantFolding pass_const_obj;
@@ -199,6 +204,41 @@ GetOutputTensor(Ort::KernelContext& context,
   return context.GetOutput(index, output_shape);
 }
 
+std::shared_ptr<OVNetwork> Set_Layout(std::shared_ptr<OVNetwork> ov_model, const layout_t& layout) {
+  ov::preprocess::PrePostProcessor preproc(ov_model);
+
+  const auto& inputs = ov_model->inputs();
+  const auto& outputs = ov_model->outputs();
+
+  auto find_tensor_index = [](const std::vector<ov::Output<ov::Node>>& tensors, const std::string& name) -> std::optional<size_t> {
+    for (size_t i = 0; i < tensors.size(); ++i) {
+      const auto& tensor = tensors[i];
+      if (tensor.get_any_name() == name || tensor.get_tensor().get_names().count(name) > 0) {
+        return i;
+      }
+    }
+    return std::nullopt;
+  };
+
+  for (const auto& [tensor_name, layout_value] : layout) {
+    bool tensor_found = false;
+
+    if (auto input_idx = find_tensor_index(inputs, tensor_name)) {
+      preproc.input(*input_idx).tensor().set_layout(layout_value);
+      tensor_found = true;
+    } else if (auto output_idx = find_tensor_index(outputs, tensor_name)) {
+      preproc.output(*output_idx).tensor().set_layout(layout_value);
+      tensor_found = true;
+    }
+
+    if (!tensor_found) {
+      LOGS_DEFAULT(WARNING) << "Tensor '" << tensor_name << "' not found in model inputs or outputs";
+    }
+  }
+
+  return preproc.build();
+}
+
 int GetFirstAvailableDevice(SessionContext& session_context) {
   int i = 0;
   // Get the first available VAD-M device and set the device to busy
diff --git a/onnxruntime/core/providers/openvino/backend_utils.h b/onnxruntime/core/providers/openvino/backend_utils.h
index 15145df651fa2..27f791c7a5bd1 100644
--- a/onnxruntime/core/providers/openvino/backend_utils.h
+++ b/onnxruntime/core/providers/openvino/backend_utils.h
@@ -79,6 +79,8 @@ int GetFirstAvailableDevice(SessionContext& session_context);
 
 void FillOutputsWithConstantData(std::shared_ptr<ov::Node> node, Ort::UnownedValue& out_tensor);
 
+std::shared_ptr<OVNetwork> Set_Layout(std::shared_ptr<OVNetwork> ov_model, const layout_t& layout);
+
 template <typename T>
 void FillOutputHelper(Ort::UnownedValue& out_tensor, std::shared_ptr<ov::Node> node);
 
diff --git a/onnxruntime/core/providers/openvino/backends/basic_backend.cc b/onnxruntime/core/providers/openvino/backends/basic_backend.cc
index 6efd866d47c3c..0f939437109a6 100644
--- a/onnxruntime/core/providers/openvino/backends/basic_backend.cc
+++ b/onnxruntime/core/providers/openvino/backends/basic_backend.cc
@@ -98,6 +98,7 @@ BasicBackend::BasicBackend(std::unique_ptr<ONNX_NAMESPACE::ModelProto>& model_pr
                                !subgraph_context_.has_dynamic_input_shape &&
                                !session_context_.so_context_enable &&
                                session_context_.reshape.empty() &&
+                               session_context_.layout.empty() &&
                                !enable_causallm &&
                                !eligible_for_cpu_fallback &&
                                auto_unified_compile);
diff --git a/onnxruntime/core/providers/openvino/contexts.h b/onnxruntime/core/providers/openvino/contexts.h
index 6a2b375d733f9..07b09899ac214 100644
--- a/onnxruntime/core/providers/openvino/contexts.h
+++ b/onnxruntime/core/providers/openvino/contexts.h
@@ -70,6 +70,7 @@ class SharedContext : public WeakSingleton<SharedContext> {
 
 using config_t = std::map<std::string, ov::AnyMap>;
 using reshape_t = std::map<std::string, ov::PartialShape>;
+using layout_t = std::map<std::string, ov::Layout>;
 
 struct ProviderInfo {
   std::string device_type{""};             // [device_type]: Overrides the accelerator hardware type and
@@ -88,6 +89,7 @@ struct ProviderInfo {
                                            // (GPU) feature. If blob files are already present,
                                            // it will be directly loaded.
   reshape_t reshape{};                     // Used for reshaping the ov input tensor shape at runtime.
+  layout_t layout{};                       // Used for specifying the ov input/output tensor layout at runtime.
   std::string model_priority{"DEFAULT"};   // High-level OpenVINO model priority hint
                                            // Defines what model should be provided with more performant
                                            // bounded resource first
@@ -110,7 +112,7 @@ struct ProviderInfo {
   const ConfigOptions* config_options{NULL};
   const std::unordered_set<std::string> valid_provider_keys = {"device_type", "device_id", "device_luid", "cache_dir", "precision",
                                                                "load_config", "context", "num_of_threads", "model_priority", "num_streams", "enable_opencl_throttling", "enable_qdq_optimizer",
-                                                               "enable_causallm", "disable_dynamic_shapes", "reshape_input"};
+                                                               "enable_causallm", "disable_dynamic_shapes", "reshape_input", "layout"};
 };
 
 // Holds context applicable to the entire EP instance.
diff --git a/onnxruntime/core/providers/openvino/openvino_parser_utils.cc b/onnxruntime/core/providers/openvino/openvino_parser_utils.cc
index 21fc7f935da23..a290fea73e0e8 100644
--- a/onnxruntime/core/providers/openvino/openvino_parser_utils.cc
+++ b/onnxruntime/core/providers/openvino/openvino_parser_utils.cc
@@ -236,5 +236,79 @@ ov::Dimension OpenVINOParserUtils::ParseDimensionRange(const std::string& range_
   return ov::Dimension(range_start, range_end);
 }
 
+layout_t OpenVINOParserUtils::ParseLayout(const std::string& layout_definition) {
+  layout_t parsed_layout_map;
+
+  // Return empty map for empty input
+  if (layout_definition.empty()) {
+    ORT_THROW("Empty layout definition provided in layout parameter");
+  }
+
+  // Regular expression for parsing layout definitions
+  const std::regex layout_pattern(R"(([^\[\],]+)\s*\[(.*?)\])");  // e.g. "input_1[NC],data[CHW]"
+
+  // Find all tensor layout definitions using regex
+  auto layout_begin = std::sregex_iterator(
+      layout_definition.begin(),
+      layout_definition.end(),
+      layout_pattern);
+  auto layout_end = std::sregex_iterator();
+
+  // If no matches found, throw error
+  if (layout_begin == layout_end) {
+    ORT_THROW("Invalid layout definition format: " + layout_definition);
+  }
+
+  // Process each tensor definition
+  for (std::sregex_iterator i = std::move(layout_begin); i != layout_end; ++i) {
+    std::smatch layout_match = *i;
+
+    // Extract tensor name and trim whitespace
+    std::string tensor_name = layout_match[1].str();  // Group 1: tensor name e.g. "input_1"
+    tensor_name = TrimWhitespace(tensor_name);
+
+    if (tensor_name.empty()) {
+      ORT_THROW("Empty tensor name provided in layout parameter");
+    }
+
+    // Extract dimensions string
+    std::string dimensions_str = layout_match[2].str();  // Group 2: dimensions string [e.g. "NC", "CHW"]
+
+    if (!Check_Valid_Layout(dimensions_str, tensor_name)) {
+      ORT_THROW("Invalid dimensions string provided in layout parameter");
+    }
+
+    // Store parsed shape in result map
+    parsed_layout_map[tensor_name] = ov::Layout(dimensions_str);
+  }
+
+  return parsed_layout_map;
+}
+
+bool OpenVINOParserUtils::Check_Valid_Layout(const std::string& layout_str, const std::string& tensor_name) {
+  // Check if the layout string is empty
+  if (layout_str.empty()) {
+    return false;
+  }
+
+  std::unordered_set<char> seen_alphabets;
+  for (char c : layout_str) {
+    if (std::isalpha(c)) {
+      char upper_c = static_cast<char>(std::toupper(c));  // Convert to uppercase for case-insensitive comparison
+      if (seen_alphabets.find(upper_c) != seen_alphabets.end()) {
+        ORT_THROW("Repeated Dim '" + std::string(1, c) +
+                  "' found in layout dimensions for tensor '" + tensor_name + "'");
+      }
+      seen_alphabets.insert(upper_c);
+    } else if (c != '?') {
+      // Only '?' is allowed as non-alphabetic character
+      ORT_THROW("Invalid character '" + std::string(1, c) +
+                "' found in layout dimensions for tensor '" + tensor_name + "'");
+    }
+  }
+
+  return true;
+}
+
 }  // namespace openvino_ep
 }  // namespace onnxruntime
diff --git a/onnxruntime/core/providers/openvino/openvino_parser_utils.h b/onnxruntime/core/providers/openvino/openvino_parser_utils.h
index e6aa0e0a46a3b..a0936d627df40 100644
--- a/onnxruntime/core/providers/openvino/openvino_parser_utils.h
+++ b/onnxruntime/core/providers/openvino/openvino_parser_utils.h
@@ -18,8 +18,10 @@ class OpenVINOParserUtils {
                                     std::string& device_type,
                                     const std::string& option_name);
   static reshape_t ParseInputShape(const std::string& reshape_input_definition);
+  static layout_t ParseLayout(const std::string& layout_definition);
   static std::string TrimWhitespace(const std::string& str);
   static ov::Dimension ParseDimensionRange(const std::string& range_str, const std::string& tensor_name);
+  static bool Check_Valid_Layout(const std::string& layout_str, const std::string& tensor_name);
 };
 
 }  // namespace openvino_ep
diff --git a/onnxruntime/core/providers/openvino/openvino_provider_factory.cc b/onnxruntime/core/providers/openvino/openvino_provider_factory.cc
index 9dba8623031d0..c7fbae324a225 100644
--- a/onnxruntime/core/providers/openvino/openvino_provider_factory.cc
+++ b/onnxruntime/core/providers/openvino/openvino_provider_factory.cc
@@ -230,6 +230,10 @@ static void ParseProviderInfo(const ProviderOptions& provider_options,
     pi.reshape = OpenVINOParserUtils::ParseInputShape(provider_options.at("reshape_input"));
   }
 
+  if (provider_options.contains("layout")) {
+    pi.layout = OpenVINOParserUtils::ParseLayout(provider_options.at("layout"));
+  }
+
   if (provider_options.contains("load_config")) {
     auto parse_config = [&](const std::string& config_str) -> std::map<std::string, ov::AnyMap> {
       // If the config string is empty, return an empty map and skip processing
diff --git a/onnxruntime/test/perftest/command_args_parser.cc b/onnxruntime/test/perftest/command_args_parser.cc
index 843875a881f0a..e992ce3304d7c 100644
--- a/onnxruntime/test/perftest/command_args_parser.cc
+++ b/onnxruntime/test/perftest/command_args_parser.cc
@@ -81,7 +81,9 @@ namespace perftest {
       "\t    [OpenVINO only] [num_of_threads]: Overrides the accelerator hardware type and precision with these values at runtime.\n"
       "\t    [OpenVINO only] [cache_dir]: Explicitly specify the path to dump and load the blobs(Model caching) or cl_cache (Kernel Caching) files feature. If blob files are already present, it will be directly loaded.\n"
       "\t    [OpenVINO only] [enable_opencl_throttling]: Enables OpenCL queue throttling for GPU device(Reduces the CPU Utilization while using GPU) \n"
-      "\t    [Example] [For OpenVINO EP] -e openvino -i \"device_type|CPU num_of_threads|5 enable_opencl_throttling|true cache_dir|\"<path>\"\"\n"
+      "\t    [OpenVINO only] [reshape_input]: Sets model input shapes with support for bounded dynamic dimensions using 'min..max' syntax (e.g., [1..10,3,224,224]) \n"
+      "\t    [OpenVINO only] [layout]: Specifies the layout for inputs/outputs to interpret tensor dimensions correctly. \n"
+      "\t    [Example] [For OpenVINO EP] -e openvino -i \"device_type|CPU num_of_threads|5 enable_opencl_throttling|true reshape_input|<input_name>[1,3,60,60..100] layout|<input_name>[NCHW] cache_dir|\"<path>\"\"\n"
       "\n"
       "\t    [QNN only] [backend_type]: QNN backend type. E.g., 'cpu', 'htp'. Mutually exclusive with 'backend_path'.\n"
       "\t    [QNN only] [backend_path]: QNN backend path. E.g., '/folderpath/libQnnHtp.so', '/winfolderpath/QnnHtp.dll'. Mutually exclusive with 'backend_type'.\n"
diff --git a/onnxruntime/test/perftest/ort_test_session.cc b/onnxruntime/test/perftest/ort_test_session.cc
index 7a210ca8482a4..da6e66bae3eb0 100644
--- a/onnxruntime/test/perftest/ort_test_session.cc
+++ b/onnxruntime/test/perftest/ort_test_session.cc
@@ -828,12 +828,14 @@ select from 'TF8', 'TF16', 'UINT8', 'FLOAT', 'ITENSOR'. \n)");
         ov_options[key] = value;
       } else if (key == "reshape_input") {
         ov_options[key] = value;
+      } else if (key == "layout") {
+        ov_options[key] = value;
       } else {
         ORT_THROW(
             "[ERROR] [OpenVINO] wrong key type entered. Choose from the following runtime key options that are available for OpenVINO."
             " ['device_type', 'device_id', 'num_of_threads', 'load_config', 'cache_dir', 'num_streams', "
             "'enable_opencl_throttling', 'disable_dynamic_shapes', 'enable_qdq_optimizer',"
-            " 'enable_causallm', 'model_priority'] \n");
+            " 'enable_causallm', 'reshape_input', 'layout', 'model_priority'] \n");
       }
     }
     session_options.AppendExecutionProvider_OpenVINO_V2(ov_options);