Error snakemake --configfile multiPrime.yaml -s multiPrime.py --cores 10 --resources mem_mb=80000

[yuzh2000]

MissingInputException in line 43 of /home/data/t220349/yuzh/multiPrime_test1/multiPrime/multiPrime.py:
Missing input files for rule seq_format:
output: yuzh/multiPrime_test1/multiPrime/results/Total_fa/1000.format.fa
wildcards: virus=1000
affected files:
yuzh/multiPrime_test1/multiPrime/test_data/1000.fa

When I run to this step, an error is reported, I installed it according to the video tutorial, may I ask what the problem is？

[joybio]

@yuzh2000
I observed that the path of your input file differs from the script path. I noticed that your present working directory (pwd) is set to

/home/data/t220349/yuzh/multiPrime_test1/multiPrime/multiPrime.py

but your input file is located at

yuzh/multiPrime_test1/multiPrime/test_data/1000.fa. 

Modifying the input file path should resolve the issue.

[joybio]

@yuzh2000
By the way, If your input sequence is relatively small, and you're seeking potential degenerate primer pairs, our recent project could be beneficial. We've developed a Windows application multiPrime2-GUI also a website https://multiPrime.cn. We would greatly appreciate your assistance in enhancing and refining this tool.

[yuzh2000]

@joybio
You are really a very nice person! Thank you very much for answering my doubts! Wish you happy every day!😊😀