diff --git a/CHANGES.rst b/CHANGES.rst
index 7a94e8f8..c5ee9875 100644
--- a/CHANGES.rst
+++ b/CHANGES.rst
@@ -5,6 +5,7 @@ Unreleased
 
 -   Drop support for Python 3.9.
 -   Remove previously deprecated code.
+-   Support Python 3.14 template strings. :issue:`511`
 
 
 Version 3.0.3
diff --git a/docs/formatting.rst b/docs/formatting.rst
index c14f9170..3a786650 100644
--- a/docs/formatting.rst
+++ b/docs/formatting.rst
@@ -6,6 +6,26 @@ String Formatting
 The :class:`Markup` class can be used as a format string. Objects
 formatted into a markup string will be escaped first.
 
+t-strings
+---------
+
+.. versionadded:: 3.1.0
+
+On Python 3.14 :ref:`python:t-strings` can be passed to :class:`Markup`
+(or :func:`escape`), any interpolated value will be escaped while literal
+content will be treated as as markup.
+
+.. code-block:: pycon
+
+    >>> uid, name = 3, "<script>"
+    >>> Markup(t'<a href="/user/{uid}">{name}</a>')
+    Markup('<a href="/user/3">&lt;script&gt;</a>')
+
+.. seealso::
+
+    - :ref:`What's new in Python 3.14: template string literals <python:whatsnew314-template-string-literals>`
+    - :pep:`750`
+    - :mod:`string.templatelib`
 
 Format Method
 -------------
diff --git a/src/markupsafe/__init__.py b/src/markupsafe/__init__.py
index f8a0d58b..3168e746 100644
--- a/src/markupsafe/__init__.py
+++ b/src/markupsafe/__init__.py
@@ -9,6 +9,12 @@
 except ImportError:
     from ._native import _escape_inner
 
+try:
+    from string.templatelib import Interpolation  # type: ignore[import-not-found]
+    from string.templatelib import Template
+except ImportError:
+    Template = Interpolation = None
+
 if t.TYPE_CHECKING:
     import typing_extensions as te
 
@@ -36,9 +42,13 @@ def escape(s: t.Any, /) -> Markup:
     # conversion. This is the most common use case.
     # Use type(s) instead of s.__class__ because a proxy object may be reporting
     # the __class__ of the proxied value.
-    if type(s) is str:
+    s_type = type(s)
+    if s_type is str:
         return Markup(_escape_inner(s))
 
+    if s_type is Template:
+        return _template__html__(s)
+
     if hasattr(s, "__html__"):
         return Markup(s.__html__())
 
@@ -122,7 +132,10 @@ class Markup(str):
     def __new__(
         cls, object: t.Any = "", encoding: str | None = None, errors: str = "strict"
     ) -> te.Self:
-        if hasattr(object, "__html__"):
+        if type(object) is Template:
+            object = _template__html__(object)
+
+        elif hasattr(object, "__html__"):
             object = object.__html__()
 
         if encoding is None:
@@ -377,3 +390,21 @@ def __int__(self, /) -> int:
 
     def __float__(self, /) -> float:
         return float(self.obj)
+
+
+def _template__html__(
+    s: Template,
+    *,
+    escape: t.Callable[[t.Any], Markup] = escape,
+    str: t.Callable[[t.Any], str] = str,
+    Markup: t.Callable[[t.Any], Markup] = Markup,
+    format: t.Callable[[t.Any, str], str] = format,
+) -> Markup:
+    return Markup("").join(
+        Markup(value)
+        if value.__class__ is str
+        else escape(value.value)
+        if value.format_spec is None
+        else escape(format(value.value, value.format_spec))
+        for value in s
+    )
diff --git a/tests/test_markupsafe.py b/tests/test_markupsafe.py
index 85d9ea16..161efc00 100644
--- a/tests/test_markupsafe.py
+++ b/tests/test_markupsafe.py
@@ -2,6 +2,12 @@
 
 import typing as t
 
+try:
+    from string.templatelib import Interpolation  # type: ignore[import-not-found]
+    from string.templatelib import Template
+except ImportError:
+    Template = Interpolation = None
+
 import pytest
 
 from markupsafe import escape
@@ -25,14 +31,31 @@ def test_adding() -> None:
             {"username": "<bad user>"},
             "<em>&lt;bad user&gt;</em>",
         ),
-        ("%i", 3.14, "3"),
-        ("%.2f", 3.14, "3.14"),
+        ("%i", 3.1415, "3"),
+        ("%.2f", 3.1415, "3.14"),
     ),
 )
 def test_string_interpolation(template: str, data: t.Any, expect: str) -> None:
     assert Markup(template) % data == expect
 
 
+@pytest.mark.skipif(Template is None, reason="requires Python 3.14+")
+@pytest.mark.parametrize(
+    ("template", "expect"),
+    (
+        (
+            lambda: Template("<em>", Interpolation("<bad user>", "username"), "</em>"),
+            "<em>&lt;bad user&gt;</em>",
+        ),
+        (lambda: Template(Interpolation(3.1415, "value", None, ".0f")), "3"),
+        (lambda: Template(Interpolation(3.1415, "value", None, ".2f")), "3.14"),
+    ),
+)
+def test_string_template(template: t.Callable[[], Template], expect: str) -> None:
+    assert Markup(template()) == expect
+    assert escape(template()) == expect
+
+
 def test_type_behavior() -> None:
     assert type(Markup("foo") + "bar") is Markup
     x = Markup("foo")