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Ambiguous bases #254

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OliverPStuart opened this issue Feb 14, 2023 · 1 comment
Open

Ambiguous bases #254

OliverPStuart opened this issue Feb 14, 2023 · 1 comment

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@OliverPStuart
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Hi there,

I am examining a wtdbg2.5 assembly and I have two contigs with short (23 bp) stretches of ambiguous N bases. This is not a problem per se but I do not understand what these Ns represent.

Can you please advise?

Best,
Ollie
@ruanjue
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ruanjue commented Feb 14, 2023

Possiblly caused by mis-joint of seqs of nearby edges, but this bug is too rare to be debuged.

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@ruanjue @OliverPStuart