From c682e3fe6acb02762d94a809151ffb6f461bc367 Mon Sep 17 00:00:00 2001 From: yukiteruono Date: Wed, 12 Feb 2025 20:48:53 +0900 Subject: [PATCH] v3.0.5 --- README.md | 1 + 1 file changed, 1 insertion(+) diff --git a/README.md b/README.md index 015163f..8371769 100644 --- a/README.md +++ b/README.md @@ -5,6 +5,7 @@ We have developed PBSIM, a simulator for all types of Pacific Biosciences (PacBi PBSIM can simulate whole genome sequencing (WGS) and transcriptome sequencing (TS) of the PacBio RS II continuous long reads (CLR), PacBio Sequel CLR, PacBio Sequel high-fidelity (HiFi) reads, and ONT reads. PBSIM does not directly simulate HiFi reads, but only simulate generation of CLR by multi-pass sequencing; the output of PBSIM simulation is input into ccs software (https://github.com/PacificBiosciences/ccs), which generates HiFi reads. In addition, PBSIM can simulate full-length sequencing of user entered templates. + Note: To compress the output files, SAMtools (https://github.com/samtools/samtools) and gzip (https://www.gnu.org/software/gzip/) must be installed in the PBSIM execution environment.