Metabolism at a glance - Assignment

Black box model

In a batch fermentation experiment:

• a 2 L fermentor was inoculated with 0.02 g DW/L of yeast.
• Initial glucose concentration was 20 g/L.

At the end of the exponential growth phase, it was found that

• the medium contained 10 g/L ethanol,
• 2.74 g DW/L biomass and
• 1.54 g/L glycerol.
• The final glucose concentration was practically 0 g/L.

In assignment.py:

1. Calculate the specific yield coefficients (C-mol) for all products.
2. Calculate the carbon balance.
3. Assuming that CO2 is the only missing product, calculate how much CO2 was produced in the fermentation.

Hint: Assume the following average biomass elemental composition (Villadsen et al., p. 74):