GC Content Analysis Workflow

This project provides a Nextflow workflow designed to analyze the GC content of DNA sequences provided in a FASTA file. It identifies sequences with GC content above a specified threshold and outputs them into a results file.

Features

• Input: Accepts a FASTA file and a GC content threshold as inputs.
• Processing: Calculates the GC content for each sequence in the input file using Biopython.
• Output: Writes sequences with GC content above the specified threshold into a file (output.txt) in a designated results directory.
• Configuration: Includes a configurable nextflow.config file for customizing the output directory and file handling.

Requirements

• Nextflow: Version 24.10.0 or later.
• Python: Ensure Python 3.x is installed with the following library:
  • Biopython: Install using pip install biopython.

Usage

1. Clone this repository:

   git clone https://github.com/yourusername/GC_Content_Analysis.git