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Hello! This is an automated update of the following workflow: workflows/amplicon/qiime2/qiime2-III-VI-downsteam. I created this PR because I think one or more of the component tools are out of date, i.e. there is a newer version available on the ToolShed.

By comparing with the latest versions available on the ToolShed, it seems the following tools are outdated:

  • toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__fragment_insertion__sepp/qiime2__fragment_insertion__sepp/2024.10.0+q2galaxy.2024.10.0 should be updated to toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__fragment_insertion__sepp/qiime2__fragment_insertion__sepp/2025.4.0+q2galaxy.2025.4.0
  • toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__feature_classifier__classify_sklearn/qiime2__feature_classifier__classify_sklearn/2024.10.0+q2galaxy.2024.10.0 should be updated to toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__feature_classifier__classify_sklearn/qiime2__feature_classifier__classify_sklearn/2025.4.0+q2galaxy.2025.4.0
  • toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__taxa__barplot/qiime2__taxa__barplot/2024.10.0+q2galaxy.2024.10.0 should be updated to toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__taxa__barplot/qiime2__taxa__barplot/2025.4.0+q2galaxy.2025.4.0
  • toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__metadata__tabulate/qiime2__metadata__tabulate/2024.10.0+q2galaxy.2024.10.0 should be updated to toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__metadata__tabulate/qiime2__metadata__tabulate/2025.4.0+q2galaxy.2025.4.0
  • toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__diversity__alpha_rarefaction/qiime2__diversity__alpha_rarefaction/2024.10.0+q2galaxy.2024.10.0 should be updated to toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__diversity__alpha_rarefaction/qiime2__diversity__alpha_rarefaction/2025.4.0+q2galaxy.2025.4.0
  • toolshed.g2.bx.psu.edu/repos/q2d2/qiime2_core__tools__import/qiime2_core__tools__import/2024.10.0+dist.h3d8a7e27 should be updated to toolshed.g2.bx.psu.edu/repos/q2d2/qiime2_core__tools__import/qiime2_core__tools__import/2025.4.0+dist.h1147124e
  • toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__diversity__core_metrics_phylogenetic/qiime2__diversity__core_metrics_phylogenetic/2024.10.0+q2galaxy.2024.10.0 should be updated to toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__diversity__core_metrics_phylogenetic/qiime2__diversity__core_metrics_phylogenetic/2025.4.0+q2galaxy.2025.4.0
  • toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__diversity__alpha_group_significance/qiime2__diversity__alpha_group_significance/2024.10.0+q2galaxy.2024.10.0 should be updated to toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__diversity__alpha_group_significance/qiime2__diversity__alpha_group_significance/2025.4.0+q2galaxy.2025.4.0
  • toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__diversity__beta_group_significance/qiime2__diversity__beta_group_significance/2024.10.0+q2galaxy.2024.10.0 should be updated to toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__diversity__beta_group_significance/qiime2__diversity__beta_group_significance/2025.4.0+q2galaxy.2025.4.0

The workflow release number has been updated from 0.2 to 0.3.

The workflow release number has been updated from 0.2 to 0.3.

If you want to skip this change, close this PR without deleting the branch. It will be reopened if another change is detected.
Any commit from another author than 'planemo-autoupdate' will prevent more auto-updates.
To ignore manual changes and allow autoupdates, delete the branch.

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Test Results (powered by Planemo)

Test Summary

Test State Count
Total 2
Passed 1
Error 0
Failure 1
Skipped 0
Failed Tests
  • ❌ QIIME2-VI-diversity-metrics-and-estimations.ga_0

    Problems:

    • Output with path /tmp/tmp1a15pp1d/beta_diversity_weighted_unifrac__3b9ba44c-451b-4228-bb03-7148adbf4cb0 different than expected
      Expected file size to be in [400k:500k] found 504807
      

    Workflow invocation details

    • Invocation Messages

    • Steps
      • Step 1: Sampling depth:

        • step_state: scheduled
      • Step 2: Metadata:

        • step_state: scheduled
      • Step 11: Beta diversity - Jaccard distance matrix:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity beta_group_significance '/tmp/tmppomf81kc/job_working_directory/000/19/configs/tmp3ptn2b4_'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_beta/_visualizer.py:174: FutureWarning: The behavior of DataFrame concatenation with empty or all-NA entries is deprecated. In a future version, this will no longer exclude empty or all-NA columns when determining the result dtypes. To retain the old behavior, exclude the relevant entries before the concat operation.
                pairs_summary = pd.concat([pairs_summary, group_pairs_summary])
              

            Standard Output:

            • 「distance_matrix: 95990253-c5d5-4924-868a-432390b1cf03」
              「metadata: <CategoricalMetadataColumn name='cage_id' id_count=48>」
              「method: 'permanova'」
              「pairwise: False」
              「permutations: 999」
                                                                                                                                                                                                                                                                             
              Saved Visualization to: visualization.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __q2galaxy__GUI__section__extra_opts__ {"method": "permanova", "pairwise": false, "permutations": "999"}
              __workflow_invocation_uuid__ "25011be22ef211f0a452000d3a17e462"
              chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata {"__current_case__": 1, "column": "cage_id", "source": {"values": [{"id": 15, "src": "hda"}]}, "type": "qza"}
      • Step 12: Beta diversity - Bray-Curtis distance matrix:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity beta_group_significance '/tmp/tmppomf81kc/job_working_directory/000/20/configs/tmpv9_nuhsq'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_beta/_visualizer.py:174: FutureWarning: The behavior of DataFrame concatenation with empty or all-NA entries is deprecated. In a future version, this will no longer exclude empty or all-NA columns when determining the result dtypes. To retain the old behavior, exclude the relevant entries before the concat operation.
                pairs_summary = pd.concat([pairs_summary, group_pairs_summary])
              

            Standard Output:

            • 「distance_matrix: 60069135-46e2-47d9-b865-f79e04ddc6a8」
              「metadata: <CategoricalMetadataColumn name='cage_id' id_count=48>」
              「method: 'permanova'」
              「pairwise: False」
              「permutations: 999」
                                                                                                                                                                                                                                                                             
              Saved Visualization to: visualization.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __q2galaxy__GUI__section__extra_opts__ {"method": "permanova", "pairwise": false, "permutations": "999"}
              __workflow_invocation_uuid__ "25011be22ef211f0a452000d3a17e462"
              chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata {"__current_case__": 1, "column": "cage_id", "source": {"values": [{"id": 15, "src": "hda"}]}, "type": "qza"}
      • Step 13: Emperor plot collection:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __workflow_invocation_uuid__ "25011be22ef211f0a452000d3a17e462"
              datasets [{"__index__": 0, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "unweighted_unifrac"}, "input": {"values": [{"id": 29, "src": "hda"}]}}, {"__index__": 1, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "weighted_unifrac"}, "input": {"values": [{"id": 30, "src": "hda"}]}}, {"__index__": 2, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "jaccard"}, "input": {"values": [{"id": 31, "src": "hda"}]}}, {"__index__": 3, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "bray_curtis"}, "input": {"values": [{"id": 32, "src": "hda"}]}}]
      • Step 14: Beta diversity - weighted UniFrac distance matrix:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity beta_group_significance '/tmp/tmppomf81kc/job_working_directory/000/22/configs/tmp3jnssos1'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_beta/_visualizer.py:174: FutureWarning: The behavior of DataFrame concatenation with empty or all-NA entries is deprecated. In a future version, this will no longer exclude empty or all-NA columns when determining the result dtypes. To retain the old behavior, exclude the relevant entries before the concat operation.
                pairs_summary = pd.concat([pairs_summary, group_pairs_summary])
              

            Standard Output:

            • 「distance_matrix: 2dd30b1c-d8f5-4ea7-9df5-f3827e1fe485」
              「metadata: <CategoricalMetadataColumn name='cage_id' id_count=48>」
              「method: 'permanova'」
              「pairwise: False」
              「permutations: 999」
                                                                                                                                                                                                                                                                             
              Saved Visualization to: visualization.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __q2galaxy__GUI__section__extra_opts__ {"method": "permanova", "pairwise": false, "permutations": "999"}
              __workflow_invocation_uuid__ "25011be22ef211f0a452000d3a17e462"
              chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata {"__current_case__": 1, "column": "cage_id", "source": {"values": [{"id": 15, "src": "hda"}]}, "type": "qza"}
      • Step 15: PCoA collection:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __workflow_invocation_uuid__ "25011be22ef211f0a452000d3a17e462"
              datasets [{"__index__": 0, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "unweighted_unifrac"}, "input": {"values": [{"id": 28, "src": "hda"}]}}, {"__index__": 1, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "weighted_unifrac"}, "input": {"values": [{"id": 26, "src": "hda"}]}}, {"__index__": 2, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "jaccard"}, "input": {"values": [{"id": 25, "src": "hda"}]}}, {"__index__": 3, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "bray_curtis"}, "input": {"values": [{"id": 27, "src": "hda"}]}}]
      • Step 16: Distance matrix collection:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __workflow_invocation_uuid__ "25011be22ef211f0a452000d3a17e462"
              datasets [{"__index__": 0, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "unweighted_unifrac"}, "input": {"values": [{"id": 24, "src": "hda"}]}}, {"__index__": 1, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "weighted_unifrac"}, "input": {"values": [{"id": 22, "src": "hda"}]}}, {"__index__": 2, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "jaccard"}, "input": {"values": [{"id": 21, "src": "hda"}]}}, {"__index__": 3, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "bray_curtis"}, "input": {"values": [{"id": 23, "src": "hda"}]}}]
      • Step 17: Richness and evenness collection:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __workflow_invocation_uuid__ "25011be22ef211f0a452000d3a17e462"
              datasets [{"__index__": 0, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "rarefied_table"}, "input": {"values": [{"id": 16, "src": "hda"}]}}, {"__index__": 1, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "faith_pd_vector"}, "input": {"values": [{"id": 17, "src": "hda"}]}}, {"__index__": 2, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "observed_features_vector"}, "input": {"values": [{"id": 18, "src": "hda"}]}}, {"__index__": 3, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "shannon_vector"}, "input": {"values": [{"id": 20, "src": "hda"}]}}, {"__index__": 4, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "eveness_vector"}, "input": {"values": [{"id": 19, "src": "hda"}]}}]
      • Step 3: Feature table:

        • step_state: scheduled
      • Step 4: Rooted tree:

        • step_state: scheduled
      • Step 5: Target metadata parameter (for beta diversity):

        • step_state: scheduled
      • Step 6: toolshed.g2.bx.psu.edu/repos/q2d2/qiime2_core__tools__import/qiime2_core__tools__import/2025.4.0+dist.h1147124e:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run tools import '/tmp/tmppomf81kc/job_working_directory/000/14/configs/tmpl7jin51m'

            Exit Code:

            • 0

            Standard Output:

            • 「type: ImmutableMetadata」
              「format: ImmutableMetadataFormat」
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "25011be22ef211f0a452000d3a17e462"
              chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              import_root {"__current_case__": 59, "__q2galaxy__GUI__cond__format__": {"__current_case__": 0, "format": "ImmutableMetadataFormat", "import": {"data": {"values": [{"id": 12, "src": "hda"}]}, "name": "__q2galaxy__::literal::None"}}, "type": "ImmutableMetadata"}
      • Step 7: Diversity metrics:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity core_metrics_phylogenetic '/tmp/tmppomf81kc/job_working_directory/000/15/configs/tmp5nw8obab'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/skbio/util/_warning.py:60: UserWarning: observed_otus is deprecated as of 0.6.0.
                warn(f"{func.__name__} is deprecated as of {ver}.")
              /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:146: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.008592360631028074 and the largest is 5.250221977897388.
                warn(
              /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:146: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.08744210267781792 and the largest is 4.83121202245088.
                warn(
              /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:146: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.02238803416531163 and the largest is 2.2154065166410293.
                warn(
              /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:146: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.028624230910658778 and the largest is 0.6164051191609862.
                warn(
              

            Standard Output:

            • 「table: 07913ecd-c005-4f93-b52c-50dcde5b2e13」
              「phylogeny: b37f57aa-ce89-466a-82e7-14a9417b8cd0」
              「sampling_depth: 2000」
              「metadata: <Metadata>」
              「with_replacement: False」
              「ignore_missing_samples: False」
                                                                                                                                                                                                                                                                             
              Running external command line application. This may print messages to stdout and/or stderr.
              The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
              
              Command:
              
              faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/500490e2-e458-40a0-826e-d90a0cfc2c4c/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/b37f57aa-ce89-466a-82e7-14a9417b8cd0/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027831.22@uid=#1001/tmp/q2-OutPath-ydt017rx
              
              Running external command line application. This may print messages to stdout and/or stderr.
              The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
              
              Command:
              
              ssu -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/500490e2-e458-40a0-826e-d90a0cfc2c4c/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/b37f57aa-ce89-466a-82e7-14a9417b8cd0/data/tree.nwk -m unweighted -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027831.22@uid=#1001/tmp/q2-OutPath-wpasdq1s
              
              Running external command line application. This may print messages to stdout and/or stderr.
              The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
              
              Command:
              
              ssu -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/500490e2-e458-40a0-826e-d90a0cfc2c4c/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/b37f57aa-ce89-466a-82e7-14a9417b8cd0/data/tree.nwk -m weighted_unnormalized -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027831.22@uid=#1001/tmp/q2-OutPath-ixxtk7be
              
              Saved FeatureTable[Frequency] to: rarefied_table.qza
              Saved SampleData[AlphaDiversity] to: faith_pd_vector.qza
              Saved SampleData[AlphaDiversity] to: observed_features_vector.qza
              Saved SampleData[AlphaDiversity] to: shannon_vector.qza
              Saved SampleData[AlphaDiversity] to: evenness_vector.qza
              Saved DistanceMatrix to: unweighted_unifrac_distance_matrix.qza
              Saved DistanceMatrix to: weighted_unifrac_distance_matrix.qza
              Saved DistanceMatrix to: jaccard_distance_matrix.qza
              Saved DistanceMatrix to: bray_curtis_distance_matrix.qza
              Saved PCoAResults to: unweighted_unifrac_pcoa_results.qza
              Saved PCoAResults to: weighted_unifrac_pcoa_results.qza
              Saved PCoAResults to: jaccard_pcoa_results.qza
              Saved PCoAResults to: bray_curtis_pcoa_results.qza
              Saved Visualization to: unweighted_unifrac_emperor.qzv
              Saved Visualization to: weighted_unifrac_emperor.qzv
              Saved Visualization to: jaccard_emperor.qzv
              Saved Visualization to: bray_curtis_emperor.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __q2galaxy__GUI__section__extra_opts__ {"ignore_missing_samples": false, "with_replacement": false}
              __workflow_invocation_uuid__ "25011be22ef211f0a452000d3a17e462"
              chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata [{"__index__": 0, "__q2galaxy__GUI__conditional__metadata__": {"__current_case__": 0, "source": {"values": [{"id": 12, "src": "hda"}]}, "type": "tsv"}}]
              sampling_depth "2000"
      • Step 8: Alpha diversity metrics - Pielou's evenness:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity alpha_group_significance '/tmp/tmppomf81kc/job_working_directory/000/16/configs/tmpr1ejlqa9'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
              

            Standard Output:

            • 「alpha_diversity: 6bc07fc4-4f3c-483c-9f1c-51bb2bbb69bc」
              「metadata: <Metadata>」
                                                                                                                                                                                                                                                                             
              Saved Visualization to: visualization.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "25011be22ef211f0a452000d3a17e462"
              chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata [{"__index__": 0, "__q2galaxy__GUI__conditional__metadata__": {"__current_case__": 0, "source": {"values": [{"id": 12, "src": "hda"}]}, "type": "tsv"}}]
      • Step 9: Alpha diversity metrics - Observed features:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity alpha_group_significance '/tmp/tmppomf81kc/job_working_directory/000/17/configs/tmpwhsebkhv'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
              

            Standard Output:

            • 「alpha_diversity: cb0c3a63-4761-47f3-8b58-492be9b76393」
              「metadata: <Metadata>」
                                                                                                                                                                                                                                                                             
              Saved Visualization to: visualization.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "25011be22ef211f0a452000d3a17e462"
              chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata [{"__index__": 0, "__q2galaxy__GUI__conditional__metadata__": {"__current_case__": 0, "source": {"values": [{"id": 12, "src": "hda"}]}, "type": "tsv"}}]
      • Step 10: Alpha diversity metrics - Shannon's diversity index:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity alpha_group_significance '/tmp/tmppomf81kc/job_working_directory/000/18/configs/tmpdbfoel11'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
              

            Standard Output:

            • 「alpha_diversity: 91db387a-4628-4532-afda-15e7c33b3412」
              「metadata: <Metadata>」
                                                                                                                                                                                                                                                                             
              Saved Visualization to: visualization.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "25011be22ef211f0a452000d3a17e462"
              chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata [{"__index__": 0, "__q2galaxy__GUI__conditional__metadata__": {"__current_case__": 0, "source": {"values": [{"id": 12, "src": "hda"}]}, "type": "tsv"}}]
    • Other invocation details
      • history_id

        • 51588883e4a923bc
      • history_state

        • ok
      • invocation_id

        • 6608fcdda181eae8
      • invocation_state

        • scheduled
      • workflow_id

        • 6608fcdda181eae8
Passed Tests
  • ✅ QIIME2-III-V-Phylogeny-Rarefaction-Taxonomic-Analysis.ga_0

    Workflow invocation details

    • Invocation Messages

    • Steps
      • Step 1: Representative sequences:

        • step_state: scheduled
      • Step 2: Feature table:

        • step_state: scheduled
      • Step 3: Metadata:

        • step_state: scheduled
      • Step 4: Minimum depth:

        • step_state: scheduled
      • Step 5: Maximum depth:

        • step_state: scheduled
      • Step 6: SEPP fragment insertion reference:

        • step_state: scheduled
      • Step 7: Taxonomic classifier:

        • step_state: scheduled
      • Step 8: Phylogenetic tree for diversity analysis:

        • step_state: scheduled

        • Subworkflow Steps
          • Step 1: DADA2 representative sequences:

            • step_state: scheduled
          • Step 2: SEPP fragment insertion source file:

            • step_state: scheduled
          • Step 3: Phylogenetic tree generation:

            • step_state: scheduled

            • Jobs
              • Job 1:

                • Job state is ok

                Command Line:

                • q2galaxy run fragment_insertion sepp '/tmp/tmppomf81kc/job_working_directory/000/6/configs/tmpksexrygp'

                Exit Code:

                • 0

                Standard Output:

                • 「representative_sequences: 455eda14-0d01-4a9b-98af-d3eaa0c0d79a」
                  「reference_database: a14c6180-506b-4ecb-bacb-9cb30bc3044b」
                  「alignment_subset_size: 1000」
                  「placement_subset_size: 5000」
                  「debug: False」
                                                                                                                                                                                                                                                                                 
                  ==> sepp-q2-fragment-insertion-err.log <==
                  [05:23:43] jobs.py (line 131):     INFO: Finished pplacer Job with input: backbone_alignment_file:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_48/pplacer.backbone.712szl08.fasta, tree_file:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_48/pplacer.tree.2yupqwfv.tre, info_file:/tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt, extended alignment:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_48/pplacer.extended.0.o56e8bpo.fasta, output:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_48/pplacer.extended.0.o56e8bpo.jplace
                  [05:23:49] jobs.py (line 131):     INFO: Finished pplacer Job with input: backbone_alignment_file:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_49/pplacer.backbone.czl0uyhb.fasta, tree_file:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_49/pplacer.tree.s_z_m1o1.tre, info_file:/tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt, extended alignment:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_49/pplacer.extended.0.auxz5gnx.fasta, output:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_49/pplacer.extended.0.auxz5gnx.jplace
                  [05:23:53] jobs.py (line 131):     INFO: Finished pplacer Job with input: backbone_alignment_file:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_50/pplacer.backbone.atc125jy.fasta, tree_file:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_50/pplacer.tree.ccue4e58.tre, info_file:/tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt, extended alignment:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_50/pplacer.extended.0.1m3gb2mu.fasta, output:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_50/pplacer.extended.0.1m3gb2mu.jplace
                  [05:23:56] jobs.py (line 131):     INFO: Finished pplacer Job with input: backbone_alignment_file:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_54/pplacer.backbone.89lhamkl.fasta, tree_file:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_54/pplacer.tree.9ydfoe6g.tre, info_file:/tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt, extended alignment:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_54/pplacer.extended.0.fymdiiwr.fasta, output:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_54/pplacer.extended.0.fymdiiwr.jplace
                  [05:23:59] jobs.py (line 131):     INFO: Finished pplacer Job with input: backbone_alignment_file:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_60/pplacer.backbone.kjvds6lj.fasta, tree_file:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_60/pplacer.tree.uk3msqad.tre, info_file:/tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt, extended alignment:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_60/pplacer.extended.0.ktg2ng69.fasta, output:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_60/pplacer.extended.0.ktg2ng69.jplace
                  [05:24:02] jobs.py (line 131):     INFO: Finished pplacer Job with input: backbone_alignment_file:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_61/pplacer.backbone.oprks7yg.fasta, tree_file:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_61/pplacer.tree.v6qqzdmt.tre, info_file:/tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt, extended alignment:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_61/pplacer.extended.0.zbwzvzxy.fasta, output:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf/root/P_61/pplacer.extended.0.zbwzvzxy.jplace
                  [05:24:37] jobs.py (line 131):     INFO: Finished jsonmerger Job with input: input:pipe output:/tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tmp-k3t2D1sUXO/q2-fragment-insertion_placement.json
                  [05:24:39] algorithm.py (line 213):     INFO: Current execution Finished in 640 seconds
                  [05:24:39] algorithm.py (line 215):     INFO: All checkpointed executions Finished in 640 cumulative time
                  [05:24:39] algorithm.py (line 224):     INFO: Temp files are left behind at /tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tempssd-uN7wJRxG50/q2-fragment-insertion.qnez9uyf
                  
                  ==> sepp-q2-fragment-insertion-out.log <==
                  root_p='/opt/conda/envs/qiime2-amplicon-2025.4/share/sepp/sepp'
                  output files are at q2-fragment-insertion_placement.json q2-fragment-insertion_placement.tog.relabelled.tre q2-fragment-insertion_placement.tog.relabelled.xml q2-fragment-insertion_placement.tog.tre q2-fragment-insertion_placement.tog.xml and more files are at /tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tmp-k3t2D1sUXO . Consider removing /tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tmp-k3t2D1sUXO if its files are not needed.
                  Removing /tmp/tmppomf81kc/tmp/tmp.u5O1AB3AgI/sepp-tmp-k3t2D1sUXO
                  Saved Phylogeny[Rooted] to: tree.qza
                  Saved Placements to: placements.qza
                  

                Traceback:

                Job Parameters:

                • Job parameter Parameter value
                  __input_ext "input"
                  __q2galaxy__GUI__section__extra_opts__ {"alignment_subset_size": "1000", "debug": false, "placement_subset_size": "5000"}
                  __workflow_invocation_uuid__ "4a60fc352eef11f0a452000d3a17e462"
                  chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
                  dbkey "?"
      • Step 9: Taxonomic analysis:

        • step_state: scheduled

        • Subworkflow Steps
          • Step 1: Metadata:

            • step_state: scheduled
          • Step 2: DADA2 representative sequences:

            • step_state: scheduled
          • Step 3: Taxonomic classifier:

            • step_state: scheduled
          • Step 4: DADA2 feature table:

            • step_state: scheduled
          • Step 5: Taxonomy classification:

            • step_state: scheduled

            • Jobs
              • Job 1:

                • Job state is ok

                Command Line:

                • q2galaxy run feature_classifier classify_sklearn '/tmp/tmppomf81kc/job_working_directory/000/7/configs/tmpd50dq9yn'

                Exit Code:

                • 0

                Standard Output:

                • 「reads: 455eda14-0d01-4a9b-98af-d3eaa0c0d79a」
                  「classifier: dc39ac42-8ecf-4093-8efa-14513ccde419」
                  「reads_per_batch: 'auto'」
                  「pre_dispatch: '2*n_jobs'」
                  「confidence: 0.7」
                  「read_orientation: 'auto'」
                                                                                                                                                                                                                                                                                 
                  Saved FeatureData[Taxonomy] to: classification.qza
                  

                Traceback:

                Job Parameters:

                • Job parameter Parameter value
                  __input_ext "input"
                  __q2galaxy__GUI__section__extra_opts__ {"__q2galaxy__GUI__conditional__confidence__": {"__current_case__": 1, "__q2galaxy__GUI__select__": "__q2galaxy__::control::Float X Range(0__comma__ 1__comma__ inclusive_end=True)", "confidence": "0.7"}, "__q2galaxy__GUI__conditional__reads_per_batch__": {"__current_case__": 0, "__q2galaxy__GUI__select__": "auto", "reads_per_batch": "auto"}, "pre_dispatch": "2*n_jobs", "read_orientation": "auto"}
                  __workflow_invocation_uuid__ "4a60fc362eef11f0a452000d3a17e462"
                  chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
                  dbkey "?"
          • Step 6: Taxonomy barplot:

            • step_state: scheduled

            • Jobs
              • Job 1:

                • Job state is ok

                Command Line:

                • q2galaxy run taxa barplot '/tmp/tmppomf81kc/job_working_directory/000/8/configs/tmpnwgvllhn'

                Exit Code:

                • 0

                Standard Output:

                • 「table: 07913ecd-c005-4f93-b52c-50dcde5b2e13」
                  「taxonomy: d4cb2706-1932-4d41-90d0-b814ce15dcee」
                  「metadata: <Metadata>」
                  「level_delimiter: None」
                                                                                                                                                                                                                                                                                 
                  Saved Visualization to: visualization.qzv
                  

                Traceback:

                Job Parameters:

                • Job parameter Parameter value
                  __input_ext "input"
                  __q2galaxy__GUI__section__extra_opts__ {"__q2galaxy__GUI__conditional__level_delimiter__": {"__current_case__": 0, "__q2galaxy__GUI__select__": "__q2galaxy__::control::default", "level_delimiter": "__q2galaxy__::literal::None"}, "metadata": [{"__index__": 0, "__q2galaxy__GUI__conditional__metadata__": {"__current_case__": 0, "source": {"values": [{"id": 1, "src": "hda"}]}, "type": "tsv"}}], "taxonomy": {"values": [{"id": 8, "src": "hda"}]}}
                  __workflow_invocation_uuid__ "4a60fc362eef11f0a452000d3a17e462"
                  chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
                  dbkey "?"
          • Step 7: Tabulate taxonomy classification:

            • step_state: scheduled

            • Jobs
              • Job 1:

                • Job state is ok

                Command Line:

                • q2galaxy run metadata tabulate '/tmp/tmppomf81kc/job_working_directory/000/9/configs/tmp4jh9sjf5'

                Exit Code:

                • 0

                Standard Output:

                • 「input: <Metadata>」
                  「page_size: 100」
                                                                                                                                                                                                                                                                                 
                  Saved Visualization to: visualization.qzv
                  

                Traceback:

                Job Parameters:

                • Job parameter Parameter value
                  __input_ext "input"
                  __q2galaxy__GUI__section__extra_opts__ {"page_size": "100"}
                  __workflow_invocation_uuid__ "4a60fc362eef11f0a452000d3a17e462"
                  chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
                  dbkey "?"
                  input [{"__index__": 0, "__q2galaxy__GUI__conditional__input__": {"__current_case__": 1, "source": {"values": [{"id": 8, "src": "hda"}]}, "type": "qza"}}]
      • Step 10: Rarefaction:

        • step_state: scheduled

        • Subworkflow Steps
          • Step 1: Metadata:

            • step_state: scheduled
          • Step 2: DADA2 feature table:

            • step_state: scheduled
          • Step 3: Rooted tree:

            • step_state: scheduled
          • Step 4: Minimum depth:

            • step_state: scheduled
          • Step 5: Maximum depth:

            • step_state: scheduled
          • Step 6: Alpha rarefaction:

            • step_state: scheduled

            • Jobs
              • Job 1:

                • Job state is ok

                Command Line:

                • q2galaxy run diversity alpha_rarefaction '/tmp/tmppomf81kc/job_working_directory/000/10/configs/tmpukr9rxu8'

                Exit Code:

                • 0

                Standard Error:

                • /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/skbio/util/_warning.py:60: UserWarning: observed_otus is deprecated as of 0.6.0.
                    warn(f"{func.__name__} is deprecated as of {ver}.")
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_num
                  ..
                  265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.4/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  

                Standard Output:

                • 「table: 07913ecd-c005-4f93-b52c-50dcde5b2e13」
                  「max_depth: 2019」
                  「phylogeny: 38884db0-e577-4364-ba8d-abde416cefc3」
                  「metrics: None」
                  「metadata: <Metadata>」
                  「min_depth: 1」
                  「steps: 10」
                  「iterations: 10」
                                                                                                                                                                                                                                                                                 
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/0646f9bd-f71e-4fa0-a481-dc35c9163107/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-24xgfy_n
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c6e5fdad-271e-4438-b5f6-f0d4825a6f13/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-eq2ohj33
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/f6c401f3-51a6-4806-9680-e492ec8c4741/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-uuyocoyw
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/ba5b16ea-7101-414f-9947-769963b1350e/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-kz218es9
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/1d68927c-8348-4795-b289-7a52becd5087/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-ro8sk5b7
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/65ede320-e7e9-4aef-b1dd-c7b9687e775b/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-8e5diu_i
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/98710f50-e60e-4444-8787-829fe5935efb/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-5k3j4v6d
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/2203765f-6de4-4844-8938-25762b4847c3/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-jxi5ukzl
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/2630e195-5a28-41ab-a55f-35f540804e66/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-dgf16fks
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/1bfada33-8f05-4624-a354-d20724cc5bab/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-g9eb9j4t
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/7d3f3b89-443d-4aae-8dac-31949ed96df5/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-wkg4p8mv
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/f50cfd01-a6b1-4f1e-abf1-4b7180adb379/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-3dl_2s18
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/a02aa697-9ac4-4b1a-b2f7-18520bf05242/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-wxlx200q
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/5187c224-fe6c-42c6-bfc2-62f83bdd00d9/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-9eulh0wo
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/41980cf4-4cb6-4336-9d9b-180a42411398/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-m8xbs3r0
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/2fdc2957-7b6d-4bf4-a4d7-f7acd6bdd480/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-2lwh1gre
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/d52a075b-0e8c-4e86-b3e6-8ba9a2c045be/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-irz11r9y
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/8b5117a6-9cf0-4659-a64d-ac7036576fa3/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-_44ihn6o
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/f8e15ab8-1955-4c01-962d-782f07718ec1/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-db2ku88c
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/b717115a-b537-402c-b4c4-cdde73f86ba9/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-w25w11do
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/1a3289ab-5945-4745-8fcc-44d71c84b44a/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-stubi5xv
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c2b06c23-3960-431d-a6da-3f09cafefd9d/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-odwd2unm
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/65221768-6463-43bb-ab5f-4d84a43a0870/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-fd380ymi
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/0a8f6876-f706-4fd8-a7df-de7926ef6aa4/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-mtc1rz2d
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/88a0f128-77e1-4772-9bf2-2192a49aeca9/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-z0__id_x
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/56271f8f-d64a-4161-88f4-a8a4e0f097b8/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-f8u_mwts
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/dd1a7cd7-aabf-447c-8887-c5ba5253d3e3/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-49wackcf
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/9b0fd72e-2c22-44b4-a385-8b49aac323d0/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-8wu_jwws
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/3db54867-2e39-4c60-bad4-4607836a36c3/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-ycue5_kx
                  
                  Runni
                  ..
                  and being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/6260680f-e700-4607-8519-0cbf3096cb28/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-mg1zur5w
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c008e8f8-4f29-4422-afd6-fb9622998485/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-fmu3q72n
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/6e8aae8d-255d-4c8d-a871-d4a4f997bc77/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-3upx37lp
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c8d271a6-10e5-4308-8b83-c4ff6dec6b18/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-v7baioym
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/0cc699b4-fa79-452a-94a6-c28846fdcf58/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-acjc_2d2
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/10da3404-f20d-47c7-bfc6-aa9c08d1f490/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-x7kdtscc
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/b2123ea9-ecb9-42d5-a0f8-148f0173ff82/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-lwym9fpf
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/8a13b81a-5130-4dc0-aebb-9c089855aa00/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-a5mkdwiu
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/202e7d12-6ec2-40ac-92e0-9559785b5270/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-3nuuplhm
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/a074ae9a-d583-4963-9df6-1fe676b5f1b2/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-jf41c9a2
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/8ce4f4f5-8178-4092-9781-a0df4ee6025e/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-n2e651kh
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/7051b24c-29f6-40b9-816a-d079b18a30ae/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-pt8lnz_l
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/907bf15e-0921-482f-95b4-7c6f1d6aa59d/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-42c9uv67
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/d717855d-47d0-45a1-ba70-760dd2fc936f/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-4fpzpkcu
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/b1521a50-9e68-4bda-8957-a724dec0cdc8/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-8uqtd9p0
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/1b8688a8-3154-4b81-ba94-d0a0a584fa87/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-011s7j32
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c170227a-7c87-4e4d-b1a6-0f5ccf2a1453/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-v2ddnhuf
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/28ec733d-23b2-468d-94b3-722c11238cf2/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-5vvwwhga
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/d4d7f9be-0279-4213-be2e-6608dd3f364b/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-29ka4q5m
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/28b80160-6720-47ab-8485-68f89a8997d4/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-sk82sbcm
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/90dbeb7a-ad2f-4bd5-ae78-4b0076820aba/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-g5osbn7d
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/ee684a84-08b3-4aba-94f3-d186c9b4273f/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-hdz1hpp0
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/8fdd80ab-1b71-42aa-957a-1f0514a8cc26/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-vhflsy2z
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/bee080ab-1121-4202-9c16-6c86f1597192/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-y8q2fpn9
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/a52514c1-a52f-4523-92aa-c035c8554f2e/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-wi3p1iq1
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/500a8859-8ac6-4614-9523-f6b17d575ad9/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-hkgof15f
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/d5f05859-179c-43ee-a747-f8b77431e9dd/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-xlhvzrh7
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/eafca7ab-a8a2-4faf-a8a5-ab5f2680218d/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-cy38gezu
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/91a53ecc-a85a-4fda-8251-f0f609d90b7c/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-eba6u6f8
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/62f3024c-2a42-44a3-8c3f-ac5a0609d0ca/data/feature-table.biom -t /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/data/c1e96f28-86f3-4bdb-b372-8694da0edda5/data/tree.nwk -o /tmp/tmppomf81kc/tmp/qiime2/uid=#1001/processes/52-1747027578.14@uid=#1001/tmp/q2-OutPath-uc1khcmt
                  
                  Saved Visualization to: visualization.qzv
                  

                Traceback:

                Job Parameters:

                • Job parameter Parameter value
                  __input_ext "input"
                  __q2galaxy__GUI__section__extra_opts__ {"iterations": "10", "metadata": [{"__index__": 0, "__q2galaxy__GUI__conditional__metadata__": {"__current_case__": 0, "source": {"values": [{"id": 1, "src": "hda"}]}, "type": "tsv"}}], "metrics": [], "min_depth": "1", "phylogeny": {"values": [{"id": 6, "src": "hda"}]}, "steps": "10"}
                  __workflow_invocation_uuid__ "4a60fc372eef11f0a452000d3a17e462"
                  chromInfo "/tmp/tmppomf81kc/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
                  dbkey "?"
                  max_depth "2019"
    • Other invocation details
      • history_id

        • a9671a3be5151c44
      • history_state

        • ok
      • invocation_id

        • a9671a3be5151c44
      • invocation_state

        • scheduled
      • workflow_id

        • 0a79a9a95ddd0a22

@gxydevbot gxydevbot changed the title Updating workflows/amplicon/qiime2/qiime2-III-VI-downsteam from 0.2 to 0.3 Updating workflows/amplicon/qiime2/qiime2-III-VI-downsteam from 0.2 to 0.3 Jul 21, 2025
@gxydevbot gxydevbot force-pushed the workflows/amplicon/qiime2/qiime2-III-VI-downsteam branch from 62ec4a3 to 287736a Compare July 21, 2025 05:12
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There are new updates, they have been integrated to the PR, check the file diff.

@gxydevbot gxydevbot force-pushed the workflows/amplicon/qiime2/qiime2-III-VI-downsteam branch from 287736a to a3bd0e2 Compare November 3, 2025 05:15
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There are new updates, they have been integrated to the PR, check the file diff.

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github-actions bot commented Nov 3, 2025

Test Results (powered by Planemo)

Test Summary

Test State Count
Total 2
Passed 1
Error 0
Failure 1
Skipped 0
Failed Tests
  • ❌ QIIME2-VI-diversity-metrics-and-estimations.ga_0

    Problems:

    • Output with path /tmp/tmpzwsbscfd/beta_diversity_weighted_unifrac__a34948ff-fff1-4894-9859-2eb3a2a35560.qzv different than expected
      Expected file size to be in [400k:500k] found 500412
      

    Workflow invocation details

    • Invocation Messages

    • Steps
      • Step 1: Sampling depth:

        • step_state: scheduled
      • Step 2: Metadata:

        • step_state: scheduled
      • Step 11: Beta diversity - Jaccard distance matrix:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity beta_group_significance '/tmp/tmp0y4ht89e/job_working_directory/000/19/configs/tmpjo9wchy_'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                import pkg_resources
              /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_beta/_visualizer.py:174: FutureWarning: The behavior of DataFrame concatenation with empty or all-NA entries is deprecated. In a future version, this will no longer exclude empty or all-NA columns when determining the result dtypes. To retain the old behavior, exclude the relevant entries before the concat operation.
                pairs_summary = pd.concat([pairs_summary, group_pairs_summary])
              

            Standard Output:

            • 「distance_matrix: d677de54-202a-475d-b8c9-b6a8293d862c」
              「metadata: <CategoricalMetadataColumn name='cage_id' id_count=48>」
              「method: 'permanova'」
              「pairwise: False」
              「permutations: 999」
                                                                                                                                                                                                                                                                             
              Saved Visualization to: visualization.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __q2galaxy__GUI__section__extra_opts__ {"method": "permanova", "pairwise": false, "permutations": "999"}
              __workflow_invocation_uuid__ "1416b164b87911f082706045bd857693"
              chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata {"__current_case__": 1, "column": "cage_id", "source": {"values": [{"id": 15, "src": "hda"}]}, "type": "qza"}
      • Step 12: Beta diversity - Bray-Curtis distance matrix:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity beta_group_significance '/tmp/tmp0y4ht89e/job_working_directory/000/20/configs/tmp0tysncsv'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                import pkg_resources
              /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_beta/_visualizer.py:174: FutureWarning: The behavior of DataFrame concatenation with empty or all-NA entries is deprecated. In a future version, this will no longer exclude empty or all-NA columns when determining the result dtypes. To retain the old behavior, exclude the relevant entries before the concat operation.
                pairs_summary = pd.concat([pairs_summary, group_pairs_summary])
              

            Standard Output:

            • 「distance_matrix: 1e8da073-6d97-4672-b523-b31f49945ac0」
              「metadata: <CategoricalMetadataColumn name='cage_id' id_count=48>」
              「method: 'permanova'」
              「pairwise: False」
              「permutations: 999」
                                                                                                                                                                                                                                                                             
              Saved Visualization to: visualization.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __q2galaxy__GUI__section__extra_opts__ {"method": "permanova", "pairwise": false, "permutations": "999"}
              __workflow_invocation_uuid__ "1416b164b87911f082706045bd857693"
              chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata {"__current_case__": 1, "column": "cage_id", "source": {"values": [{"id": 15, "src": "hda"}]}, "type": "qza"}
      • Step 13: Emperor plot collection:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __workflow_invocation_uuid__ "1416b164b87911f082706045bd857693"
              datasets [{"__index__": 0, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "unweighted_unifrac"}, "input": {"values": [{"id": 29, "src": "hda"}]}}, {"__index__": 1, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "weighted_unifrac"}, "input": {"values": [{"id": 30, "src": "hda"}]}}, {"__index__": 2, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "jaccard"}, "input": {"values": [{"id": 31, "src": "hda"}]}}, {"__index__": 3, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "bray_curtis"}, "input": {"values": [{"id": 32, "src": "hda"}]}}]
      • Step 14: Beta diversity - weighted UniFrac distance matrix:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity beta_group_significance '/tmp/tmp0y4ht89e/job_working_directory/000/22/configs/tmpv4351_y6'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                import pkg_resources
              /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_beta/_visualizer.py:174: FutureWarning: The behavior of DataFrame concatenation with empty or all-NA entries is deprecated. In a future version, this will no longer exclude empty or all-NA columns when determining the result dtypes. To retain the old behavior, exclude the relevant entries before the concat operation.
                pairs_summary = pd.concat([pairs_summary, group_pairs_summary])
              

            Standard Output:

            • 「distance_matrix: af0a153e-b393-42bb-8ec4-89aae1d3a03f」
              「metadata: <CategoricalMetadataColumn name='cage_id' id_count=48>」
              「method: 'permanova'」
              「pairwise: False」
              「permutations: 999」
                                                                                                                                                                                                                                                                             
              Saved Visualization to: visualization.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __q2galaxy__GUI__section__extra_opts__ {"method": "permanova", "pairwise": false, "permutations": "999"}
              __workflow_invocation_uuid__ "1416b164b87911f082706045bd857693"
              chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata {"__current_case__": 1, "column": "cage_id", "source": {"values": [{"id": 15, "src": "hda"}]}, "type": "qza"}
      • Step 15: PCoA collection:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __workflow_invocation_uuid__ "1416b164b87911f082706045bd857693"
              datasets [{"__index__": 0, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "unweighted_unifrac"}, "input": {"values": [{"id": 28, "src": "hda"}]}}, {"__index__": 1, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "weighted_unifrac"}, "input": {"values": [{"id": 26, "src": "hda"}]}}, {"__index__": 2, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "jaccard"}, "input": {"values": [{"id": 25, "src": "hda"}]}}, {"__index__": 3, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "bray_curtis"}, "input": {"values": [{"id": 27, "src": "hda"}]}}]
      • Step 16: Distance matrix collection:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __workflow_invocation_uuid__ "1416b164b87911f082706045bd857693"
              datasets [{"__index__": 0, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "unweighted_unifrac"}, "input": {"values": [{"id": 24, "src": "hda"}]}}, {"__index__": 1, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "weighted_unifrac"}, "input": {"values": [{"id": 22, "src": "hda"}]}}, {"__index__": 2, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "jaccard"}, "input": {"values": [{"id": 21, "src": "hda"}]}}, {"__index__": 3, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "bray_curtis"}, "input": {"values": [{"id": 23, "src": "hda"}]}}]
      • Step 17: Richness and evenness collection:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __workflow_invocation_uuid__ "1416b164b87911f082706045bd857693"
              datasets [{"__index__": 0, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "rarefied_table"}, "input": {"values": [{"id": 16, "src": "hda"}]}}, {"__index__": 1, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "faith_pd_vector"}, "input": {"values": [{"id": 17, "src": "hda"}]}}, {"__index__": 2, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "observed_features_vector"}, "input": {"values": [{"id": 18, "src": "hda"}]}}, {"__index__": 3, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "shannon_vector"}, "input": {"values": [{"id": 20, "src": "hda"}]}}, {"__index__": 4, "id_cond": {"__current_case__": 2, "id_select": "manual", "identifier": "eveness_vector"}, "input": {"values": [{"id": 19, "src": "hda"}]}}]
      • Step 3: Feature table:

        • step_state: scheduled
      • Step 4: Rooted tree:

        • step_state: scheduled
      • Step 5: Target metadata parameter (for beta diversity):

        • step_state: scheduled
      • Step 6: toolshed.g2.bx.psu.edu/repos/q2d2/qiime2_core__tools__import/qiime2_core__tools__import/2025.10.0+dist.hbfe50fe0:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run tools import '/tmp/tmp0y4ht89e/job_working_directory/000/14/configs/tmpk7v4a8wy'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                import pkg_resources
              

            Standard Output:

            • 「type: ImmutableMetadata」
              「format: ImmutableMetadataFormat」
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "1416b164b87911f082706045bd857693"
              chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              import_root {"__current_case__": 60, "__q2galaxy__GUI__cond__format__": {"__current_case__": 0, "format": "ImmutableMetadataFormat", "import": {"data": {"values": [{"id": 12, "src": "hda"}]}, "name": "__q2galaxy__::literal::None"}}, "type": "ImmutableMetadata"}
      • Step 7: Diversity metrics:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity core_metrics_phylogenetic '/tmp/tmp0y4ht89e/job_working_directory/000/15/configs/tmpludlfac3'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                import pkg_resources
              /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/skbio/util/_warning.py:60: UserWarning: observed_otus is deprecated as of 0.6.0.
                warn(f"{func.__name__} is deprecated as of {ver}.")
              /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:146: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.014050117860068526 and the largest is 5.236370619671905.
                warn(
              /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:146: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.09627599647650634 and the largest is 4.823923621467573.
                warn(
              /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:146: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.02100377325056434 and the largest is 2.2247891102080692.
                warn(
              /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:146: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.028362743674163932 and the largest is 0.6174229274392956.
                warn(
              

            Standard Output:

            • 「table: 07913ecd-c005-4f93-b52c-50dcde5b2e13」
              「phylogeny: b37f57aa-ce89-466a-82e7-14a9417b8cd0」
              「sampling_depth: 2000」
              「metadata: <Metadata>」
              「with_replacement: False」
              「ignore_missing_samples: False」
                                                                                                                                                                                                                                                                             
              Running external command line application. This may print messages to stdout and/or stderr.
              The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
              
              Command:
              
              faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/15/tmp/qiime2/uid=#1001/processes/51-1762149100.4@uid=#1001/tmp/q2-OutPath-deigmgz3 -t /tmp/tmp0y4ht89e/job_working_directory/000/15/tmp/qiime2/uid=#1001/data/b37f57aa-ce89-466a-82e7-14a9417b8cd0/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/15/tmp/qiime2/uid=#1001/processes/51-1762149100.4@uid=#1001/tmp/q2-OutPath-q8k4hyhc
              
              Running external command line application. This may print messages to stdout and/or stderr.
              The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
              
              Command:
              
              ssu -i /tmp/tmp0y4ht89e/job_working_directory/000/15/tmp/qiime2/uid=#1001/data/c7e7b5f4-69bc-4fd1-b03c-133682881a41/data/feature-table.biom -t /tmp/tmp0y4ht89e/job_working_directory/000/15/tmp/qiime2/uid=#1001/data/b37f57aa-ce89-466a-82e7-14a9417b8cd0/data/tree.nwk -m unweighted -o /tmp/tmp0y4ht89e/job_working_directory/000/15/tmp/qiime2/uid=#1001/processes/51-1762149100.4@uid=#1001/tmp/q2-OutPath-yc506zn1
              
              Running external command line application. This may print messages to stdout and/or stderr.
              The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
              
              Command:
              
              ssu -i /tmp/tmp0y4ht89e/job_working_directory/000/15/tmp/qiime2/uid=#1001/data/c7e7b5f4-69bc-4fd1-b03c-133682881a41/data/feature-table.biom -t /tmp/tmp0y4ht89e/job_working_directory/000/15/tmp/qiime2/uid=#1001/data/b37f57aa-ce89-466a-82e7-14a9417b8cd0/data/tree.nwk -m weighted_unnormalized -o /tmp/tmp0y4ht89e/job_working_directory/000/15/tmp/qiime2/uid=#1001/processes/51-1762149100.4@uid=#1001/tmp/q2-OutPath-iswyozpf
              
              Saved FeatureTable[Frequency] to: rarefied_table.qza
              Saved SampleData[AlphaDiversity] to: faith_pd_vector.qza
              Saved SampleData[AlphaDiversity] to: observed_features_vector.qza
              Saved SampleData[AlphaDiversity] to: shannon_vector.qza
              Saved SampleData[AlphaDiversity] to: evenness_vector.qza
              Saved DistanceMatrix to: unweighted_unifrac_distance_matrix.qza
              Saved DistanceMatrix to: weighted_unifrac_distance_matrix.qza
              Saved DistanceMatrix to: jaccard_distance_matrix.qza
              Saved DistanceMatrix to: bray_curtis_distance_matrix.qza
              Saved PCoAResults to: unweighted_unifrac_pcoa_results.qza
              Saved PCoAResults to: weighted_unifrac_pcoa_results.qza
              Saved PCoAResults to: jaccard_pcoa_results.qza
              Saved PCoAResults to: bray_curtis_pcoa_results.qza
              Saved Visualization to: unweighted_unifrac_emperor.qzv
              Saved Visualization to: weighted_unifrac_emperor.qzv
              Saved Visualization to: jaccard_emperor.qzv
              Saved Visualization to: bray_curtis_emperor.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __q2galaxy__GUI__section__extra_opts__ {"ignore_missing_samples": false, "with_replacement": false}
              __workflow_invocation_uuid__ "1416b164b87911f082706045bd857693"
              chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata [{"__index__": 0, "__q2galaxy__GUI__conditional__metadata__": {"__current_case__": 0, "source": {"values": [{"id": 12, "src": "hda"}]}, "type": "tsv"}}]
              sampling_depth "2000"
      • Step 8: Alpha diversity metrics - Pielou's evenness:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity alpha_group_significance '/tmp/tmp0y4ht89e/job_working_directory/000/16/configs/tmprgn9wd7n'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                import pkg_resources
              /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
              

            Standard Output:

            • 「alpha_diversity: 24c9f9a1-844d-45fb-adce-654e594d4388」
              「metadata: <Metadata>」
                                                                                                                                                                                                                                                                             
              Saved Visualization to: visualization.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "1416b164b87911f082706045bd857693"
              chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata [{"__index__": 0, "__q2galaxy__GUI__conditional__metadata__": {"__current_case__": 0, "source": {"values": [{"id": 12, "src": "hda"}]}, "type": "tsv"}}]
      • Step 9: Alpha diversity metrics - Observed features:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity alpha_group_significance '/tmp/tmp0y4ht89e/job_working_directory/000/17/configs/tmputudjicf'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                import pkg_resources
              /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
              

            Standard Output:

            • 「alpha_diversity: a6667e53-1553-46ec-ab84-ab1e429babd0」
              「metadata: <Metadata>」
                                                                                                                                                                                                                                                                             
              Saved Visualization to: visualization.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "1416b164b87911f082706045bd857693"
              chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata [{"__index__": 0, "__q2galaxy__GUI__conditional__metadata__": {"__current_case__": 0, "source": {"values": [{"id": 12, "src": "hda"}]}, "type": "tsv"}}]
      • Step 10: Alpha diversity metrics - Shannon's diversity index:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • q2galaxy run diversity alpha_group_significance '/tmp/tmp0y4ht89e/job_working_directory/000/18/configs/tmp0j_cojhz'

            Exit Code:

            • 0

            Standard Error:

            • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                import pkg_resources
              /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
              

            Standard Output:

            • 「alpha_diversity: 7972693f-2f36-4ea6-88f7-0394deff7919」
              「metadata: <Metadata>」
                                                                                                                                                                                                                                                                             
              Saved Visualization to: visualization.qzv
              

            Traceback:

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "1416b164b87911f082706045bd857693"
              chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              metadata [{"__index__": 0, "__q2galaxy__GUI__conditional__metadata__": {"__current_case__": 0, "source": {"values": [{"id": 12, "src": "hda"}]}, "type": "tsv"}}]
    • Other invocation details
      • history_id

        • e7725f972ab9f222
      • history_state

        • ok
      • invocation_id

        • 452b4d9797dcfd9d
      • invocation_state

        • scheduled
      • workflow_id

        • 452b4d9797dcfd9d
Passed Tests
  • ✅ QIIME2-III-V-Phylogeny-Rarefaction-Taxonomic-Analysis.ga_0

    Workflow invocation details

    • Invocation Messages

    • Steps
      • Step 1: Representative sequences:

        • step_state: scheduled
      • Step 2: Feature table:

        • step_state: scheduled
      • Step 3: Metadata:

        • step_state: scheduled
      • Step 4: Minimum depth:

        • step_state: scheduled
      • Step 5: Maximum depth:

        • step_state: scheduled
      • Step 6: SEPP fragment insertion reference:

        • step_state: scheduled
      • Step 7: Taxonomic classifier:

        • step_state: scheduled
      • Step 8: Phylogenetic tree for diversity analysis:

        • step_state: scheduled

        • Subworkflow Steps
          • Step 1: DADA2 representative sequences:

            • step_state: scheduled
          • Step 2: SEPP fragment insertion source file:

            • step_state: scheduled
          • Step 3: Phylogenetic tree generation:

            • step_state: scheduled

            • Jobs
              • Job 1:

                • Job state is ok

                Command Line:

                • q2galaxy run fragment_insertion sepp '/tmp/tmp0y4ht89e/job_working_directory/000/6/configs/tmprsq9ldtn'

                Exit Code:

                • 0

                Standard Error:

                • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                    import pkg_resources
                  

                Standard Output:

                • 「representative_sequences: 455eda14-0d01-4a9b-98af-d3eaa0c0d79a」
                  「reference_database: a14c6180-506b-4ecb-bacb-9cb30bc3044b」
                  「alignment_subset_size: 1000」
                  「placement_subset_size: 5000」
                  「debug: False」
                                                                                                                                                                                                                                                                                 
                  ==> sepp-q2-fragment-insertion-err.log <==
                  [05:44:27] jobs.py (line 131):     INFO: Finished pplacer Job with input: backbone_alignment_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_48/pplacer.backbone.bjel9rga.fasta, tree_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_48/pplacer.tree.mudl3zks.tre, info_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/qiime2/uid=#1001/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt, extended alignment:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_48/pplacer.extended.0.cyviet1c.fasta, output:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_48/pplacer.extended.0.cyviet1c.jplace
                  [05:44:33] jobs.py (line 131):     INFO: Finished pplacer Job with input: backbone_alignment_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_49/pplacer.backbone.gn7oqhrd.fasta, tree_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_49/pplacer.tree.ga83cwiz.tre, info_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/qiime2/uid=#1001/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt, extended alignment:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_49/pplacer.extended.0.sud6mafq.fasta, output:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_49/pplacer.extended.0.sud6mafq.jplace
                  [05:44:38] jobs.py (line 131):     INFO: Finished pplacer Job with input: backbone_alignment_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_50/pplacer.backbone._abr_p7k.fasta, tree_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_50/pplacer.tree.maofbwqy.tre, info_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/qiime2/uid=#1001/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt, extended alignment:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_50/pplacer.extended.0.v6wlcm6t.fasta, output:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_50/pplacer.extended.0.v6wlcm6t.jplace
                  [05:44:41] jobs.py (line 131):     INFO: Finished pplacer Job with input: backbone_alignment_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_54/pplacer.backbone.0f7ple4e.fasta, tree_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_54/pplacer.tree.uv3o0gh0.tre, info_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/qiime2/uid=#1001/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt, extended alignment:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_54/pplacer.extended.0.mv_8k863.fasta, output:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_54/pplacer.extended.0.mv_8k863.jplace
                  [05:44:44] jobs.py (line 131):     INFO: Finished pplacer Job with input: backbone_alignment_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_60/pplacer.backbone.ytvkwmbh.fasta, tree_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_60/pplacer.tree.9o90u4rj.tre, info_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/qiime2/uid=#1001/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt, extended alignment:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_60/pplacer.extended.0.u42ay1zm.fasta, output:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_60/pplacer.extended.0.u42ay1zm.jplace
                  [05:44:47] jobs.py (line 131):     INFO: Finished pplacer Job with input: backbone_alignment_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_61/pplacer.backbone.6qcszha2.fasta, tree_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_61/pplacer.tree.sw1mcgxi.tre, info_file:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/qiime2/uid=#1001/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt, extended alignment:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_61/pplacer.extended.0.54f7h81s.fasta, output:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi/root/P_61/pplacer.extended.0.54f7h81s.jplace
                  [05:45:24] jobs.py (line 131):     INFO: Finished jsonmerger Job with input: input:pipe output:/tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tmp-UQnWjAQLcz/q2-fragment-insertion_placement.json
                  [05:45:25] algorithm.py (line 213):     INFO: Current execution Finished in 652 seconds
                  [05:45:25] algorithm.py (line 215):     INFO: All checkpointed executions Finished in 652 cumulative time
                  [05:45:25] algorithm.py (line 224):     INFO: Temp files are left behind at /tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tempssd-XUnDVQBNJF/q2-fragment-insertion.in6kcoxi
                  
                  ==> sepp-q2-fragment-insertion-out.log <==
                  root_p='/opt/conda/envs/qiime2-amplicon-2025.10/share/sepp/sepp'
                  output files are at q2-fragment-insertion_placement.json q2-fragment-insertion_placement.tog.relabelled.tre q2-fragment-insertion_placement.tog.relabelled.xml q2-fragment-insertion_placement.tog.tre q2-fragment-insertion_placement.tog.xml and more files are at /tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tmp-UQnWjAQLcz . Consider removing /tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tmp-UQnWjAQLcz if its files are not needed.
                  Removing /tmp/tmp0y4ht89e/job_working_directory/000/6/tmp/tmp.uxCDzOBHLP/sepp-tmp-UQnWjAQLcz
                  Saved Phylogeny[Rooted] to: tree.qza
                  Saved Placements to: placements.qza
                  

                Traceback:

                Job Parameters:

                • Job parameter Parameter value
                  __input_ext "input"
                  __q2galaxy__GUI__section__extra_opts__ {"alignment_subset_size": "1000", "debug": false, "placement_subset_size": "5000"}
                  __workflow_invocation_uuid__ "463d1ed9b87611f082706045bd857693"
                  chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
                  dbkey "?"
      • Step 9: Taxonomic analysis:

        • step_state: scheduled

        • Subworkflow Steps
          • Step 1: Metadata:

            • step_state: scheduled
          • Step 2: DADA2 representative sequences:

            • step_state: scheduled
          • Step 3: Taxonomic classifier:

            • step_state: scheduled
          • Step 4: DADA2 feature table:

            • step_state: scheduled
          • Step 5: Taxonomy classification:

            • step_state: scheduled

            • Jobs
              • Job 1:

                • Job state is ok

                Command Line:

                • q2galaxy run feature_classifier classify_sklearn '/tmp/tmp0y4ht89e/job_working_directory/000/7/configs/tmp6wjg9uqi'

                Exit Code:

                • 0

                Standard Error:

                • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                    import pkg_resources
                  

                Standard Output:

                • 「reads: 455eda14-0d01-4a9b-98af-d3eaa0c0d79a」
                  「classifier: dc39ac42-8ecf-4093-8efa-14513ccde419」
                  「reads_per_batch: 'auto'」
                  「pre_dispatch: '2*n_jobs'」
                  「confidence: 0.7」
                  「read_orientation: 'auto'」
                                                                                                                                                                                                                                                                                 
                  Saved FeatureData[Taxonomy] to: classification.qza
                  

                Traceback:

                Job Parameters:

                • Job parameter Parameter value
                  __input_ext "input"
                  __q2galaxy__GUI__section__extra_opts__ {"__q2galaxy__GUI__conditional__confidence__": {"__current_case__": 1, "__q2galaxy__GUI__select__": "__q2galaxy__::control::Float X Range(0__comma__ 1__comma__ inclusive_end=True)", "confidence": "0.7"}, "__q2galaxy__GUI__conditional__reads_per_batch__": {"__current_case__": 0, "__q2galaxy__GUI__select__": "auto", "reads_per_batch": "auto"}, "pre_dispatch": "2*n_jobs", "read_orientation": "auto"}
                  __workflow_invocation_uuid__ "463d1edab87611f082706045bd857693"
                  chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
                  dbkey "?"
          • Step 6: Taxonomy barplot:

            • step_state: scheduled

            • Jobs
              • Job 1:

                • Job state is ok

                Command Line:

                • q2galaxy run taxa barplot '/tmp/tmp0y4ht89e/job_working_directory/000/8/configs/tmph3vn2sem'

                Exit Code:

                • 0

                Standard Error:

                • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                    import pkg_resources
                  

                Standard Output:

                • 「table: 07913ecd-c005-4f93-b52c-50dcde5b2e13」
                  「taxonomy: c712f1c1-4c4b-49d6-a126-b78e4b10a361」
                  「metadata: <Metadata>」
                  「level_delimiter: None」
                                                                                                                                                                                                                                                                                 
                  Saved Visualization to: visualization.qzv
                  

                Traceback:

                Job Parameters:

                • Job parameter Parameter value
                  __input_ext "input"
                  __q2galaxy__GUI__section__extra_opts__ {"__q2galaxy__GUI__conditional__level_delimiter__": {"__current_case__": 0, "__q2galaxy__GUI__select__": "__q2galaxy__::control::default", "level_delimiter": "__q2galaxy__::literal::None"}, "metadata": [{"__index__": 0, "__q2galaxy__GUI__conditional__metadata__": {"__current_case__": 0, "source": {"values": [{"id": 1, "src": "hda"}]}, "type": "tsv"}}], "taxonomy": {"values": [{"id": 8, "src": "hda"}]}}
                  __workflow_invocation_uuid__ "463d1edab87611f082706045bd857693"
                  chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
                  dbkey "?"
          • Step 7: Tabulate taxonomy classification:

            • step_state: scheduled

            • Jobs
              • Job 1:

                • Job state is ok

                Command Line:

                • q2galaxy run metadata tabulate '/tmp/tmp0y4ht89e/job_working_directory/000/9/configs/tmp20f7ck9j'

                Exit Code:

                • 0

                Standard Error:

                • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                    import pkg_resources
                  

                Standard Output:

                • 「input: <Metadata>」
                  「page_size: 100」
                                                                                                                                                                                                                                                                                 
                  Saved Visualization to: visualization.qzv
                  

                Traceback:

                Job Parameters:

                • Job parameter Parameter value
                  __input_ext "input"
                  __q2galaxy__GUI__section__extra_opts__ {"page_size": "100"}
                  __workflow_invocation_uuid__ "463d1edab87611f082706045bd857693"
                  chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
                  dbkey "?"
                  input [{"__index__": 0, "__q2galaxy__GUI__conditional__input__": {"__current_case__": 1, "source": {"values": [{"id": 8, "src": "hda"}]}, "type": "qza"}}]
      • Step 10: Rarefaction:

        • step_state: scheduled

        • Subworkflow Steps
          • Step 1: Metadata:

            • step_state: scheduled
          • Step 2: DADA2 feature table:

            • step_state: scheduled
          • Step 3: Rooted tree:

            • step_state: scheduled
          • Step 4: Minimum depth:

            • step_state: scheduled
          • Step 5: Maximum depth:

            • step_state: scheduled
          • Step 6: Alpha rarefaction:

            • step_state: scheduled

            • Jobs
              • Job 1:

                • Job state is ok

                Command Line:

                • q2galaxy run diversity alpha_rarefaction '/tmp/tmp0y4ht89e/job_working_directory/000/10/configs/tmpq2d289og'

                Exit Code:

                • 0

                Standard Error:

                • /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/emperor/__init__.py:9: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
                    import pkg_resources
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/skbio/util/_warning.py:60: UserWarning: observed_otus is deprecated as of 0.6.0.
                    warn(f"{func.__name__} is deprecated as of {ver}.")
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing `errors` and catch exceptions explicitly instead
                    df[cols] = df[cols].apply(pd.to_numeric, errors='ignore')
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_types/sample_data/_deferred_setup/_transformers.py:28: FutureWarning: errors='ignore' is 
                  ..
                  ntation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:260: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    summary_df = data.stack(level=0)
                  /opt/conda/envs/qiime2-amplicon-2025.10/lib/python3.10/site-packages/q2_diversity/_alpha/_visualizer.py:265: FutureWarning: The previous implementation of stack is deprecated and will be removed in a future version of pandas. See the What's New notes for pandas 2.1.0 for details. Specify future_stack=True to adopt the new implementation and silence this warning.
                    stacked_counts = counts.stack(level=0)
                  

                Standard Output:

                • 「table: 07913ecd-c005-4f93-b52c-50dcde5b2e13」
                  「max_depth: 2019」
                  「phylogeny: 43226d2c-06cc-46da-8b44-b86c672b8599」
                  「metrics: None」
                  「metadata: <Metadata>」
                  「min_depth: 1」
                  「steps: 10」
                  「iterations: 10」
                                                                                                                                                                                                                                                                                 
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-jo67rfwx -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-l0j10y61
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-6dntp4k7 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-la42pq9o
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-h9qg_4rk -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-sq9nmgd5
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-bdx5sd8q -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-w7ripqho
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-t5y52h3w -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-05bvy0y6
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-q3kfpo5s -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-pzlu92cy
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-hzo0pbgd -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-rgd__q2n
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-iem_eb3i -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-n6ws7r4d
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-kxl37t32 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-g0a2082b
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-io5uydmr -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-lins1bno
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-govcf3ih -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-ztka9fwx
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-dkmtox94 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-tlf_gphy
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-hnbpzjdy -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-ow2zc23s
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-cxc3c_w9 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-p38oizdo
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-lb8fiagv -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-p3pgkz62
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-1dk1h0pl -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-1p4znbaj
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-e9coocr7 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-p6cokvdo
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-94d6lnip -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-cgmdbx24
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-wq2xzyqw -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-mpgaaql7
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-r_eedl8t -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-zssvywpl
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-qil1idb0 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-p6a1lnxm
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-vzsut9s0 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-958qn_iw
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-rrtuxt6p -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-5_gxoeeq
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-cj2swn0y -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-i9tccuv6
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-y4cv976s -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-re83gi1h
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be 
                  ..
                  n. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-lsczsb50 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-xxrit16q
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-7iand0d4 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-zjjesl3t
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-6tn2z2f_ -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-mjx_5_59
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-m93xxbql -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-4z1fs23a
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-60l7bqez -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-luc3opjg
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-dc0g62_6 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-ohg1vlxq
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-7a_7to7k -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-yo0m4now
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-ffatsb0d -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-748oglv6
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-ddb62t2w -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-b9zqu1qi
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-q3yhsy9j -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-kfwyipux
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-bxrmzlp5 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-2_cxmj9v
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-ep81f04f -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-cj41jk_u
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-fj9wgleg -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-656p1_rg
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-zawwohks -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-44vfo9t5
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-b2tjxim3 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-upc03skk
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-_op6i3vr -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-5ooeazoa
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-imjlaj6p -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-8swmnzus
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-fjab4yyv -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-k8hmpmdn
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-qyqtyzbz -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-cw1ljb2i
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-d9wag7w3 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-gp9u7si7
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-72ywl10m -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-kcc723ha
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-tysyz6wu -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-lo5jsjbk
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-67u0adg8 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-9jlwdc_m
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-e4bz92r2 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-0wfpe5z_
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-e7digd42 -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-o_c793c8
                  
                  Running external command line application. This may print messages to stdout and/or stderr.
                  The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
                  
                  Command:
                  
                  faithpd -i /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-vw_rmzku -t /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/data/5037723f-1910-457b-b340-d5cb7699ff7a/data/tree.nwk -o /tmp/tmp0y4ht89e/job_working_directory/000/10/tmp/qiime2/uid=#1001/processes/52-1762148842.72@uid=#1001/tmp/q2-OutPath-w4tu1kew
                  
                  Saved Visualization to: visualization.qzv
                  

                Traceback:

                Job Parameters:

                • Job parameter Parameter value
                  __input_ext "input"
                  __q2galaxy__GUI__section__extra_opts__ {"iterations": "10", "metadata": [{"__index__": 0, "__q2galaxy__GUI__conditional__metadata__": {"__current_case__": 0, "source": {"values": [{"id": 1, "src": "hda"}]}, "type": "tsv"}}], "metrics": [], "min_depth": "1", "phylogeny": {"values": [{"id": 6, "src": "hda"}]}, "steps": "10"}
                  __workflow_invocation_uuid__ "463d1edbb87611f082706045bd857693"
                  chromInfo "/tmp/tmp0y4ht89e/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
                  dbkey "?"
                  max_depth "2019"
    • Other invocation details
      • history_id

        • d44510d08886bb2f
      • history_state

        • ok
      • invocation_id

        • d44510d08886bb2f
      • invocation_state

        • scheduled
      • workflow_id

        • 59ca5dd33ab8a58a

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