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The transcript numbers don't match between example_raw_talon.gtf and example_talon_abundance.tsv #151

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dudududu12138 opened this issue Jun 3, 2024 · 0 comments

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@dudududu12138
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Hi,
I ran talon with your example data and followed the wiki code(https://github.com/mortazavilab/TALON/tree/master/example). But I compare the transcripts bewteen example_raw_talon.gtf and example_talon_abundance.tsv. There are 2 more transcripts in example_raw_talon.gtf while example_talon_abundance.tsv lacks them. By the way, I generated gtf file without whitelist file.And I didn't filter the abundance file. So the transcripts should be consistent with the database file example_talon.db. Below are my codes:

talon_abundance \
       --db example_talon.db \
       -a SIRV_annot \
       --build SIRV \
       --o example

talon_create_GTF \
       --db example_talon.db \
       -a SIRV_annot \
       --build SIRV \
       --o example_raw

Below are the abundance file and gtf files:
example_talon_abundance.tsv.txt
example_raw_talon.gtf.txt
Below are the 2 transcripts that reported by gtf file while lacked in abundance file:
1717388862481(1)

Below are the screenshot of gene SIRV7.1 in example_talon_abundance.tsv. Colunm 4 is the transcript ID while SIRV7.1-SIRV705 is missing.
1717388935527

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