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Hi,
I ran talon with your example data and followed the wiki code(https://github.com/mortazavilab/TALON/tree/master/example). But I compare the transcripts bewteen example_raw_talon.gtf and example_talon_abundance.tsv. There are 2 more transcripts in example_raw_talon.gtf while example_talon_abundance.tsv lacks them. By the way, I generated gtf file without whitelist file.And I didn't filter the abundance file. So the transcripts should be consistent with the database file example_talon.db. Below are my codes:
talon_abundance \
--db example_talon.db \
-a SIRV_annot \
--build SIRV \
--o example
talon_create_GTF \
--db example_talon.db \
-a SIRV_annot \
--build SIRV \
--o example_raw
Hi,
I ran talon with your example data and followed the wiki code(https://github.com/mortazavilab/TALON/tree/master/example). But I compare the transcripts bewteen
example_raw_talon.gtf
andexample_talon_abundance.tsv
. There are 2 more transcripts inexample_raw_talon.gtf
whileexample_talon_abundance.tsv
lacks them. By the way, I generated gtf file without whitelist file.And I didn't filter the abundance file. So the transcripts should be consistent with the database fileexample_talon.db
. Below are my codes:Below are the abundance file and gtf files:
example_talon_abundance.tsv.txt
example_raw_talon.gtf.txt
Below are the 2 transcripts that reported by gtf file while lacked in abundance file:
Below are the screenshot of gene
SIRV7.1
inexample_talon_abundance.tsv
. Colunm 4 is the transcript ID whileSIRV7.1-SIRV705
is missing.The text was updated successfully, but these errors were encountered: