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@@ -5,9 +5,9 @@ cd wtdbg2 && make | |
# assemble PacBio reads | ||
./wtdbg2 -t 16 -i pacbio.fa.gz -fo prefix -L 5000 | ||
# assemble Nanopore reads | ||
./wtdbg2 -t 16 -i ont.fa.gz -fo prefix | ||
./wtdbg2 -t 16 -i ont.fa.gz -fo prefix -L 5000 | ||
# derive consensus | ||
./wtpoa-cns -t 16 -i prefix.ctg.lay > prefix.ctg.lay.fa | ||
./wtpoa-cns -t 16 -i prefix.ctg.lay -fo prefix.ctg.lay.fa | ||
``` | ||
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## <a name="intro"></a>Introduction | ||
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@@ -41,7 +41,7 @@ produces the final consensus in FASTA. A typical workflow looks like this: | |
./wtpoa-cns -t 16 -i prefix.ctg.lay -fo prefix.ctg.lay.fa | ||
``` | ||
where `-t` specifies the number of CPU cores (`-t 0` to use all processors). When the default doesn't work | ||
well, you may need to apply more options. | ||
well, you may need to apply more options. See [README-ori.md][readme-ori] for more help. | ||
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||
Wtdbg2 combines normal k-mers and homopolymer-compressed (HPC) k-mers to find | ||
read overlaps. Option `-k` specifies the length of normal k-mers, while `-p` | ||
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@@ -58,7 +58,7 @@ the assembly step (not including the consensus step): | |
|Dataset |Genome|Coverage |Wtdbg2 options|CPU hours|Peak RAM| | ||
|:-----------------------|-----:|---------:|:-------------|--------:|-------:| | ||
|[E. coli][pbcr] |4.6Mb |PacBio x20| | | | | ||
|[C. elegans][ce] |100Mb |PacBio x80| | | | | ||
|[C. elegans][ce] |100Mb |PacBio x80|-L5000 -e4 |3.3 | 9.7G| | ||
|[Human CHM1][chm1] |3Gb |PacBio x60|-L5000 -e4 |378.5 | 252.7G| | ||
|[Human NA12878][na12878]|3Gb |ONT x30 |-S2 -e2 |197.4 | 244.9G| | ||
|[Axolotl][axosra] |32Gb |PacBio x32|-L5000 -AS2 |3189.7 | 1593.6G| | ||
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@@ -76,6 +76,7 @@ the assembly step (not including the consensus step): | |
Please use the [GitHub's Issues page][issue] if you have questions. You may | ||
also directly contact Jue Ruan at [email protected]. | ||
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[readme-ori]: https://github.com/ruanjue/wtdbg2/README-ori.md | ||
[miniasm]: https://github.com/lh3/miniasm | ||
[canu]: https://github.com/marbl/canu | ||
[falcon]: https://github.com/PacificBiosciences/FALCON | ||
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