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{ | ||
"object_id": "https://raw.githubusercontent.com/HadleyKing/bco-tool/1.1.0/tests/collapsed-cwl-blastn-homologs.json", | ||
"etag": "f37a6806e8e5979b3e1527af258df96a4af116247727f8d1ad8bf2ba6173bece", | ||
"spec_version": "https://w3id.org/ieee/ieee-2791-schema/2791object.json", | ||
"provenance_domain": { | ||
"name": "HCV1a BLASTN homologue identification, with collapsed secondary files.", | ||
"version": "1.0", | ||
"derived_from": "https://github.com/HadleyKing/bco-tool/1.1.0/tests/cwl-blastn-homologs.json", | ||
"obsolete_after": "2118-09-26T14:43:43-0400", | ||
"embargo": { | ||
"start_time": "2021-08-13T10:45:22-0500", | ||
"end_time": "2021-08-13T10:45:22-0500" | ||
}, | ||
"created": "2021-08-13T10:45:22-0500", | ||
"modified": "2021-08-16T13:33:54.527692", | ||
"contributors": [ | ||
{ | ||
"name": "Charles Hadley King", | ||
"affiliation": "George Washington University", | ||
"email": "[email protected]", | ||
"contribution": [ | ||
"curatedBy" | ||
], | ||
"orcid": "https://orcid.org/0000-0003-1409-4549" | ||
}, | ||
{ | ||
"name": "Alex Coleman", | ||
"affiliation": "George Washington University", | ||
"email": "[email protected]", | ||
"contribution": [ | ||
"createdBy" | ||
], | ||
"orcid": "https://orcid.org/0000-0003-3465-0557" | ||
}, | ||
{ | ||
"name": "Raja Mazumder", | ||
"affiliation": "George Washington University", | ||
"email": "[email protected]", | ||
"contribution": [ | ||
"authoredBy" | ||
], | ||
"orcid": "https://orcid.org/0000-0001-8823-9945" | ||
} | ||
], | ||
"license": "https://creativecommons.org/licenses/by/4.0/legalcode.txt" | ||
}, | ||
"usability_domain": [ | ||
"Run blastn to identify homologs. This is an essentual 'Hello, World!' for bioinformatics. The input file is a HCV 1a isolate H77 complete genome [taxid:63746] (nucinput.fa) nucleotide sequence. The database (nucdb.fa) is a collection of HCV sequences.", | ||
"Step 1. Make blast database: makeblastdb -in nucdb.fa -dbtype nucl.", | ||
"Step 2. Run nucleotide blast blastn -db nucdb.fa -query nucinput.fa -out result." | ||
], | ||
"extension_domain": [ | ||
{ | ||
"extension_schema": "https://w3id.org/biocompute/extension_domain/1.1.0/scm/scm_extension.json", | ||
"scm_extension": { | ||
"scm_repository": "https://github.com/hadleyking/bcotool", | ||
"scm_type": "git", | ||
"scm_commit": "aeb0121c392f02364563dd57c19dbefd8b4948f6", | ||
"scm_path": "/tests/blast/blastn-homologs.cwl", | ||
"scm_preview": "https://github.com/HadleyKing/bcotool/blob/aeb0121c392f02364563dd57c19dbefd8b4948f6/tests/blast/blastn-homologs.cwl" | ||
} | ||
} | ||
], | ||
"description_domain": { | ||
"keywords": [ | ||
"HCV1a", | ||
"Common Workflow Language", | ||
"BLASTN", | ||
"homologue" | ||
], | ||
"xref": [ | ||
{ | ||
"namespace": "taxonomy", | ||
"name": "Taxonomy", | ||
"ids": [ | ||
"63746" | ||
], | ||
"access_time": "2021-01-15T10:10:50-0500" | ||
}, | ||
{ | ||
"namespace": "ena.embl", | ||
"name": "ENA", | ||
"ids": [ | ||
"M67463.1" | ||
], | ||
"access_time": "2021-01-15T10:10:50-0500" | ||
} | ||
], | ||
"platform": [ | ||
"CWL" | ||
], | ||
"pipeline_steps": [ | ||
{ | ||
"step_number": 1, | ||
"name": "makeblastdb", | ||
"description": "Make blast database", | ||
"version": "Nucleotide-Nucleotide BLAST 2.11.0+", | ||
"input_list": [ | ||
{ | ||
"uri": "https://raw.githubusercontent.com/HadleyKing/bco-tool/main/tests/blast/HepC-DB.fasta", | ||
"access_time": "2021-08-13T11:25:40-0500" | ||
} | ||
], | ||
"output_list": [ | ||
{ | ||
"uri": "file://./M67463-HepC.fa.nsq", | ||
"access_time": "2021-08-13T10:53:18-0500" | ||
}, | ||
{ | ||
"uri": "file://./M67463-HepC.fa.nin", | ||
"access_time": "2021-08-13T10:53:18-0500" | ||
}, | ||
{ | ||
"uri": "file://./M67463-HepC.fa.nhr", | ||
"access_time": "2021-08-13T10:53:18-0500" | ||
} | ||
] | ||
}, | ||
{ | ||
"step_number": 2, | ||
"name": "blastn", | ||
"description": "Run nucleotide blast", | ||
"version": "1.3", | ||
"input_list": [ | ||
{ | ||
"uri": "https://raw.githubusercontent.com/HadleyKing/bco-tool/main/tests/blast/M67463-HepC.fa", | ||
"access_time": "2021-08-13T10:53:18-0500" | ||
}, | ||
{ | ||
"uri": "file://./M67463-HepC.fa.nsq", | ||
"access_time": "2021-08-13T10:53:18-0500" | ||
}, | ||
{ | ||
"uri": "file://./M67463-HepC.fa.nin", | ||
"access_time": "2021-08-13T10:53:18-0500" | ||
}, | ||
{ | ||
"uri": "file://./M67463-HepC.fa.nhr", | ||
"access_time": "2021-08-13T10:53:18-0500" | ||
} | ||
], | ||
"output_list": [ | ||
{ | ||
"uri": "file://./blast-out.txt", | ||
"access_time": "2021-08-13T10:53:18-0500" | ||
} | ||
] | ||
} | ||
] | ||
}, | ||
"execution_domain": { | ||
"script": [ | ||
{ | ||
"uri": { | ||
"uri": "https://raw.githubusercontent.com/HadleyKing/bco-tool/main/tests/blast/blastn.cwl" | ||
} | ||
}, | ||
{ | ||
"uri": { | ||
"uri": "https://raw.githubusercontent.com/HadleyKing/bco-tool/main/tests/blast/makeblastdb.cwl" | ||
} | ||
}, | ||
{ | ||
"uri": { | ||
"uri": "https://raw.githubusercontent.com/HadleyKing/bco-tool/main/tests/blast/blastn-homologs.yml" | ||
} | ||
}, | ||
{ | ||
"uri": { | ||
"uri": "https://raw.githubusercontent.com/HadleyKing/bco-tool/main/tests/blast/blastn-homologs.cwl" | ||
} | ||
} | ||
], | ||
"script_driver": "cwltool", | ||
"software_prerequisites": [ | ||
{ | ||
"name": "cwltool", | ||
"version": "3.0.20201121085451", | ||
"uri": { | ||
"uri": "https://github.com/common-workflow-language/cwltool.git" | ||
} | ||
}, | ||
{ | ||
"name": "BLAST", | ||
"version": "2.11.0+", | ||
"uri": { | ||
"uri": "https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/" | ||
} | ||
} | ||
], | ||
"external_data_endpoints": [ | ||
{ | ||
"name": "GitHub", | ||
"url": "https://raw.githubusercontent.com/" | ||
}, | ||
{ | ||
"name": "access to ftp", | ||
"url": "https://ftp.ncbi.nlm.nih.gov/blast/executables" | ||
} | ||
], | ||
"environment_variables": { | ||
"HOSTTYPE": "x86_64-linux", | ||
"EDITOR": "vim" | ||
} | ||
}, | ||
"parametric_domain": [ | ||
{ | ||
"param": "-dbtype", | ||
"value": "nucl", | ||
"step": "1" | ||
}, | ||
{ | ||
"param": "outputBinding", | ||
"value": "glob: $(inputs.database.basename)", | ||
"step": "1" | ||
} | ||
], | ||
"io_domain": { | ||
"input_subdomain": [ | ||
{ | ||
"uri": { | ||
"filename": "HepC-DB", | ||
"uri": "https://raw.githubusercontent.com/HadleyKing/bco-tool/main/tests/blast/HepC-DB.fasta" | ||
} | ||
}, | ||
{ | ||
"uri": { | ||
"filename": "M67463-HepC", | ||
"uri": "https://raw.githubusercontent.com/HadleyKing/bco-tool/main/tests/blast/M67463-HepC.fa" | ||
} | ||
} | ||
], | ||
"output_subdomain": [ | ||
{ | ||
"mediatype": "text", | ||
"uri": { | ||
"uri": "file://./blast-out.txt", | ||
"access_time": "2021-08-13T10:53:27-0500" | ||
} | ||
} | ||
] | ||
}, | ||
"error_domain": { | ||
"empirical_error": { | ||
"Database": { | ||
"sequences": 12, | ||
"total letters": 37763 | ||
}, | ||
"Query": { | ||
"accession": "M67463.1", | ||
"length": 9416, | ||
"name": "Hepatitis C virus subtype 1a, complete genome" | ||
}, | ||
"Sequences producing significant alignments": { | ||
"NC_038882.1:1-9416 Hepatitis C virus (isolate H77) genotype 1, co...": { | ||
"Score": "16879", | ||
"E value": "0.0" | ||
}, | ||
"NC_004102.1:1-9416 Hepatitis C virus genotype 1, complete genome": { | ||
"Score": "16857", | ||
"E value": "0.0" | ||
}, | ||
"NC_009825.1:1-2495 Hepatitis C virus genotype 4, genome": { | ||
"Score": "1251", | ||
"E value": "0.0" | ||
}, | ||
"NC_009824.1:16-2609 Hepatitis C virus genotype 3, genome": { | ||
"Score": "1123", | ||
"E value": "0.0" | ||
}, | ||
"NC_009827.1:3211-7143 Hepatitis C virus genotype 6, complete genome": { | ||
"Score": "1075", | ||
"E value": "0.0" | ||
}, | ||
"NC_030791.1:1-1073 Hepatitis C virus genotype 7, complete genome": { | ||
"Score": "1068", | ||
"E value": "0.0" | ||
}, | ||
"NC_009827.1:1-896 Hepatitis C virus genotype 6, complete genome": { | ||
"Score": "1061", | ||
"E value": "0.0" | ||
}, | ||
"NC_009826.1:1-908 Hepatitis C virus genotype 5, genome": { | ||
"Score": "1035", | ||
"E value": "0.0" | ||
}, | ||
"NC_009823.1:6-892 Hepatitis C virus genotype 2, complete genome": { | ||
"Score": "1024", | ||
"E value": "0.0" | ||
}, | ||
"NC_009824.1:3292-6176 Hepatitis C virus genotype 3, genome": { | ||
"Score": "542", | ||
"E value": "2e-155" | ||
}, | ||
"NC_009824.1:7618-9385 Hepatitis C virus genotype 3, genome": { | ||
"Score": "518", | ||
"E value": "4e-148" | ||
}, | ||
"NC_009823.1:7942-9433 Hepatitis C virus genotype 2, complete genome": { | ||
"Score": "383", | ||
"E value": "1e-107" | ||
} | ||
} | ||
}, | ||
"algorithmic_error": { | ||
"Expect_threshold": "<0.001" | ||
} | ||
} | ||
} |
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